INFLUENCE OF INCREASED EXTRACELLULAR LEUCINE ON THE PROTEIN METABOLIC RESPONSES DURING OSMOTIC STRESS IN SKELETAL MUSCLE
dc.contributor.author | Dunbar, Brittany | |
dc.date.accessioned | 2016-02-18T15:10:28Z | |
dc.date.available | 2016-02-18T15:10:28Z | |
dc.identifier.uri | http://hdl.handle.net/10464/8419 | |
dc.description.abstract | The purpose of this study was to examine the effects of increased extracellular leucine concentration on protein metabolism in skeletal muscle cells when exposed to 3 different osmotic stresses. L6 skeletal muscle cells were incubated in either a normal or supplemental leucine (1.5mM) medium set to hypo-osmotic (230 ± 10 Osm), iso-osmotic (330 ± 10 Osm) or hyper-osmotic (440 ± 10 Osm) conditions. 3H-tyrosine was used to quantify protein synthesis. Western blotting analysis was performed to determine the activation of mTOR, p70S6k, ubiquitin, actin, and μ-calpain. Hypo-osmotic stress resulted in the greatest increase in protein synthesis rate under the normal-leucine condition while iso-osmotic stress has the greatest increase under the elevated-leucine condition. Elevated-leucine condition had a decreased rate in protein degradation over the normal condition within the ubiquitin proteasome pathway (p<0.05). Leucine and hypo-osmotic stress therefore creates a favourable environment for anabolic events to occur. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Brock University | en_US |
dc.subject | Protein Synthesis | en_US |
dc.subject | Protein Degradation | en_US |
dc.subject | Leucine | en_US |
dc.subject | Osmolality | en_US |
dc.subject | Skeletal Muscle | en_US |
dc.title | INFLUENCE OF INCREASED EXTRACELLULAR LEUCINE ON THE PROTEIN METABOLIC RESPONSES DURING OSMOTIC STRESS IN SKELETAL MUSCLE | en_US |
dc.type | Electronic Thesis or Dissertation | en_US |
dc.degree.name | M.Sc. Applied Health Sciences | en_US |
dc.degree.level | Masters | en_US |
dc.contributor.department | Applied Health Sciences Program | en_US |
dc.degree.discipline | Faculty of Applied Health Sciences | en_US |
refterms.dateFOA | 2021-08-03T02:25:56Z |