Browsing Earth Sciences by Author "You, Minsheng"
Ecological Risk Assessment of Soil Heavy Metals and Pesticide Residues in Tea PlantationsHe, Haifang; Shi, Longqing; Yang, Guang; You, Minsheng; Vasseur, Liette (MDPI, 2020)Tea plantations have used many synthetic chemicals to ensure performance and control of pests. This has led to increased contamination of soils and reduced tea growth. We assessed the levels of heavy metals, including Cd, Cr, Pb, Cu, Ni, Zn, Hg, As, and pesticide residues, such as HCHs, biphenyl chrysanthemum ester, methamidophos, imidacloprid, permethrin, in the soil of tea plantations of Taiwan, Tibet, Guangdong, and Fujian. The Potential Ecological Risk Index and the Nemerow comprehensive pollution index were used to analyze the data. The results showed that risk indices in Tibet, Guangdong and Fuzhou were considered as moderate ecological harm level. Ecological risk assessment index of Anxi organic and Anxi conventional tea gardens suggested a "low" risk level. The Nemerow comprehensive pollution indices for soil pesticide residues in the tea plantations of Taiwan, Tibet, Anxi organic and Anxi conventional were considered mild. Guangdong and Fuzhou had values suggesting "slight pollution” levels. According to National Soil Environmental Quality Standard (GB15618-1995), soil in tea plantations in Taiwan, Tibet, and Anxi conventional matched the national first grade of soil quality and those from Guangdong, Fuzhou, and Anxi organic tea garden matched the national second grade.
Host Plant-Derived miRNAs Potentially Modulate the Development of a Cosmopolitan Insect Pest, Plutella xylostellaZhang, Ling-Ling; JIng, Xiao-Dong; Chen, Wei; Wang, Yue; Lin, Jun-Han; Zheng, Ling; Dong, Yu-Hong; Zhou, Li; Li, Fei-Fei; Yang, Fei-Ying; et al. (MDPI, 2019)Plant microRNAs (miRNAs) have recently been reported to be involved in the cross-kingdom regulation of specific cellular and physiological processes in animals. However, little of this phenomenon is known for the communication between host plant and insect herbivore. In this study, the plant-derived miRNAs in the hemolymph of a cruciferous specialist were identified by small RNAs sequencing. A total of 39 miRNAs with typical characteristics of plant miRNAs were detected, of which 24 had read counts ≥ 2 in each library. Three plant-derived miRNAs with the highest read counts were validated, and all of them were predicted to target the hemocyanin domains-containing genes of . The luciferase assays in the S2 cell demonstrated that miR159a and novel-7703-5p could target and respectively, possibly in an incomplete complementary pairing mode. We further found that treatment with agomir-7703-5p significantly influenced the pupal development and egg-hatching rate when reared on the artificial...