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dc.contributor.authorOrmerod, Kiel G.
dc.contributor.authorRogasevskaia, Tatiana P.+-
dc.contributor.authorCoorseen, Jens R.
dc.contributor.authorMercier, A. Joffre
dc.date.accessioned2013-10-15T19:42:11Z
dc.date.available2013-10-15T19:42:11Z
dc.date.issued2012-05-20
dc.identifier.citationOrmerod KG, Rogasevskaia TP, Coorssen JR, Mercier AJ (2012) Cholesterol-Independent Effects of Methyl-b-Cyclodextrin on Chemical Synapses. PLoS ONE 7(5): e36395. doi:10.1371/journal.pone.0036395en_US
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10464/5072
dc.description.abstractThe cholesterol chelating agent, methyl-b-cyclodextrin (MbCD), alters synaptic function in many systems. At crayfish neuromuscular junctions, MbCD is reported to reduce excitatory junctional potentials (EJPs) by impairing impulse propagation to synaptic terminals, and to have no postsynaptic effects. We examined the degree to which physiological effects of MbCD correlate with its ability to reduce cholesterol, and used thermal acclimatization as an alternative method to modify cholesterol levels. MbCD impaired impulse propagation and decreased EJP amplitude by 40% (P,0.05) in preparations from crayfish acclimatized to 14uC but not from those acclimatized to 21uC. The reduction in EJP amplitude in the cold-acclimatized group was associated with a 49% reduction in quantal content (P,0.05). MbCD had no effect on input resistance in muscle fibers but decreased sensitivity to the neurotransmitter L-glutamate in both warm- and coldacclimatized groups. This effect was less pronounced and reversible in the warm-acclimatized group (90% reduction in cold, P,0.05; 50% reduction in warm, P,0.05). MbCD reduced cholesterol in isolated nerve and muscle from cold- and warmacclimatized groups by comparable amounts (nerve: 29% cold, 25% warm; muscle: 20% cold, 18% warm; P,0.05). This effect was reversed by cholesterol loading, but only in the warm-acclimatized group. Thus, effects of MbCD on glutamatesensitivity correlated with its ability to reduce cholesterol, but effects on impulse propagation and resulting EJP amplitude did not. Our results indicate that MbCD can affect both presynaptic and postsynaptic properties, and that some effects of MbCD are unrelated to cholesterol chelation.en_US
dc.language.isoenen_US
dc.publisherPLoSen_US
dc.titleCholesterol-Independent Effects of Methyl-b- Cyclodextrin on Chemical Synapsesen_US
dc.typeArticleen_US
refterms.dateFOA2021-08-07T02:21:05Z


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