Show simple item record

dc.contributor.authorTiedtke, Esther
dc.date.accessioned2013-04-15T19:40:39Z
dc.date.available2013-04-15T19:40:39Z
dc.date.issued2013-04-15
dc.identifier.urihttp://hdl.handle.net/10464/4297
dc.description.abstractMost human genes undergo alternative splicing and loss of splicing fidelity is associated with disease. Epigenetic silencing of hMLH 1 via promoter cytosine methylation is causally linked to a subset of sporadic non-polyposis colon cancer and is reversible by 5-aza-2' -deoxycytidine treatment. Here I investigated changes in hMLHI mRNA splicing profiles in normal fibroblasts and colon cancer-derived human cell lines. I established the types and frequencies of hMLHI mRNA transcripts generated under baseline conditions, after hydrogen peroxide induced oxidative stress, and in acutely 5-aza-2' -deoxycytidine-treated and stably derepressed cancer cell lines. I found that hMLHI is extensively spliced under all conditions including baseline (50% splice variants), the splice variant distribution changes in response to oxidative stress, and certain splice variants are sensitive to 5- aza-2' -deoxycytidine treatment: Splice variant diversity and frequency of exon 17 skipping correlates with the level of hMLHI promoter methylation suggesting a link between promoter methylation and mRNA splicing.en_US
dc.publisherBrock Universityen_US
dc.subjectGene splicingen_US
dc.subjectDiseaseen_US
dc.subjectColon canceren_US
dc.titleA Survey of hMLH1 mRNA Splicing Profiles in Human Cell Lines: Comparing Primary Cultured Fibroblasts Before and After Oxidative Stress and Transiently versus Stably Demethylated Colon Cancer Derived Cell Linesen_US
dc.typeElectronic Thesis or Dissertationen_US
dc.degree.nameM.Sc. Biological Sciencesen_US
dc.degree.levelMastersen_US
dc.contributor.departmentDepartment of Biological Sciencesen_US
dc.degree.disciplineFaculty of Mathematics and Scienceen_US
dc.embargo.termsNoneen_US
refterms.dateFOA2021-08-08T01:58:56Z


Files in this item

Thumbnail
Name:
Brock_Tiedtke_Esther_2011.pdf
Size:
2.665Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record