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dc.contributor.authorFajardo, Val Andrew
dc.date.accessioned2011-10-24T19:56:48Z
dc.date.available2011-10-24T19:56:48Z
dc.date.issued2011-10-24
dc.identifier.urihttp://hdl.handle.net/10464/3431
dc.description.abstractMembrane lipid composition, which includes phospholipid (PL) headgroup, and fatty acid (FA) saturation, has been shown to affect cellular function. The sarcolemma (SL) membrane is integral to skeletal muscle function and health. Previous studies assessing SL lipid composition are limited as they have 1) restricted analysis to a PL level and neglected FA composition and 2) relied on aggressive membrane isolation procedures resulting in t-tubule and sarcoplasmic reticulum contamination and unknown levels of nuclear and mitochondrial contamination. Thus, to overcome these limitations, this study assessed a method of individually skinned skeletal muscle fibres as an alternative to analyze complete sarcolemmal membrane lipid composition. The major findings of this study were 1) complete SL lipid composition can be obtained 2) the SL had higher sphingomyelin content than previous studies and 3) the SL membrane had minimal nuclear and mitochondrial contamination and was void of contamination from sarcoplasmic reticulum and t-tubules.en_US
dc.language.isoengen_US
dc.publisherBrock Universityen_US
dc.subjectmembraneen_US
dc.subjectphospholipiden_US
dc.subjectfatty aciden_US
dc.titleSarcolemmal lipid analysis from mechanically skinned rat muscle fibres.en_US
dc.typeElectronic Thesis or Dissertationen_US
dc.degree.nameM.Sc. Applied Health Sciencesen_US
dc.degree.levelMastersen_US
dc.contributor.departmentApplied Health Sciences Programen_US
dc.degree.disciplineFaculty of Applied Health Sciencesen_US
refterms.dateFOA2021-08-07T02:48:13Z


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