Browsing Ph.D. Biotechnology by Title
Now showing items 1-20 of 34
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The Arabidopsis NPR1 Protein Is a Receptor for the Plant Defense Hormone Salicylic Acid
Systemic Acquired Resistance (SAR) is a type of plant systemic resistance occurring against a broad spectrum of pathogens. It can be activated in response to pathogen infection in the model plant Arabidopsis thaliana and ... -
Considerations for the Development and Optimization of Wine made from Partially Dehydrated Grapes in Ontario, Canada
The appassimento process for making wine can mitigate climatic challenges associated with cool climate winemaking, as fruit is dried post-harvest, reducing vintage-to-vintage variation due to varying fruit quality. Resultant ... -
The Elucidation of the involvement of endonuclease DNase y in reducing DNA transfection efficiency in mammalian cells
(Brock University, 2005-05-28)Gene therapy is predicated upon efficient gene transfer. While viral vectors are the method of choice for transformation efficiency, the immunogenicity and safety concerns remain problematic. Non-viral vectors, on the ... -
Enantiodivergent chemoenzymatic synthesis of codeine
(Brock University, 2009-05-28)The present thesis describes our latest results in the chemistry of morphine alkaloids. An enantiodivergent synthesis of codeine utilizing a cis-cyclohexadiene diol derived from microbial whole cell oxidation of ... -
Evaluation of genetic and strain specific factors on root colonization in endophytic insect pathogenic fungi (EIPF) Metarhizium and Beauveria, with special emphasis on hydrophobins
The Ascomycete genera, Metarhizium and Beauveria, are traditionally known as insect pathogenic fungi and are widely used as mycopesticides in agricultural settings. More recently, an additional role of these fungi in nature ... -
An exploratory study for the discovery of non-invasive hepatocellular carcinoma biomarkers among high-risk hepatitis C virus infected patients
(Brock University, 2009-01-28)Hepatocellular Carcinoma (HCC) is a major healthcare problem, representing the third most common cause of cancer-related mortality worldwide. Chronic infections with Hepatitis B virus (HBV) and/or Hepatitis C virus (HCV) ... -
Functional Characterization of Monoterpenoid Indole Alkaloid (MIA) Biosynthetic Genes in Catharanthus roseus
(Brock University, 2013-08-23)The monoterpenoid indole alkaloids (MIAs) of Madagascar periwinkle (Catharanthus roseus) are known to be among the most important source of natural drugs used in various cancer chemotherapies. MIAs are derived by combining ... -
Functional genomics of monoterpenoid indole alkaloid biosynthesis in Rauvolfia serpentina
Monoterpenoid indole alkaloids (MIAs) are a large and heterogeneous group of nitrogen-containing specialized metabolites produced by plants belonging to the Apocynaceae, Loganiaceae and Rubiaceae families. Many of these ... -
Icewine fermentation by Saccharomyces cervisiae : fundamental stress responses and comparative fermentation dynamics
(2012-12-13)Icewine is a unique dessert wine fermented from the juice of grapes that have frozen naturally on the vine. Fermentations of 40 Brix (>400g/L soluble solids) juice typically used in Canada to produce Icewine are ... -
Investigation of the effect of microwave irradiation (2.45 GHz) on biological systems at constant bulk temperature
In this thesis the effect of MW irradiation (2.45 GHz) at constant bulk temperature was investigated on several biological systems. Studies on enzymatic activity revealed that MW irradiation could enhance the activity ... -
Large-scale studies and biophysical analysis of systems involved in plant immunity.
The field of plant immunity has progressed significantly in the last decade, driven primarily by both forward and reverse genetics and to a lesser extent by molecular biology techniques. However, many unknowns still remain ... -
The Ligand and Membrane-binding Behaviour of the Phosphatidylinositol Transfer Proteins (PITPa & PITPb)
Human Class I phosphatidylinositol transfer proteins (PITPs) exists in two forms: PITPα and PITPβ. PITPs are believed to be lipid transfer proteins based on their capacity to transfer either phosphatidylinositol (PI) or ... -
Mechanism of tocopherol transfer by human α-tocopherol transfer protein (α-hTTP)
(Brock University, 2010-10-26)Vitamin E is a well known fat soluble chain breaking antioxidant. It is a general tenn used to describe a family of eight stereoisomers of tocopherols. Selective retention of a-tocopherol in the human circulation ... -
The Molecular Consequences of CK2-mediated Phosphorylation of the TGA2 Transcription Factor within Systemic Acquired Resistance of Arabidopsis thaliana
(Brock University, 2014-08-05)During infection, the model plant Arabidopsis thaliana is capable of activating long lasting defence responses both in tissue directly affected by the pathogen and in more distal tissue. Systemic acquired resistance (SAR) ... -
Novel glutathione disulfide transferase function of CC-glutaredoxins involved in disease resistance and flower development
(Brock University, 2014-08-05)Glutaredoxins are oxidoreductases capable of reducing protein disulfide bridges and glutathione mixed disulfides through the process of deglutathionylation and glutathionylation. Lately, redox-mediated modifications of ... -
Novel plant DNA binding protein: Non-expresser of pathogenesis related 1 gene (NPR1) involved in disease resistance
For the first time, through validation of the modified Chromatin Immunoprecipitation (ChIP) method (in vitro ChIP), the direct binding of NPR1 to the PR1 promoter was demonstrated. This is a novel advancement on plant ... -
ORGANOSILICON BIOTECHNOLOGY: A BIO-INSPIRED APPROACH TO THE HYDROLYSIS OF ALKOXYSILANES and THE LIPASE-CATALYZED SYNTHESIS OF SILOXANE-CONTAINING POLYESTERS AND POLYAMIDES
(Brock University, 2013-02-22)The first part of this thesis studied the capacity of amino acids and enzymes to catalyze the hydrolysis and condensation of tetraethoxysilane and phenyltrimethoxysilane. Selected amino acids were shown to accelerate the ... -
The osmoadaptive response of the wine yeast Saccharomyces cerevisiae K1-V1116 during icewine fermentation
(Brock University, 2008-05-28)The adapted metabolic response of commercial wine yeast under prolonged exposure to concentrated solutes present in Icewine juice is not fully understood. Presently, there is no information regarding the transcriptomic ... -
Plant rhizosphere specificity and variability in the insect and plant adhesins, Madl and Mad2, within the genus Metarhizium suggest plant adaptation as an evolutionary force
(2013-04-19)Metarhizium is a soil-inhabiting fungus currently used as a biological control agent against various insect species, and research efforts are typically focused on its ability to kill insects. In section 1, we tested the ... -
The production and synthetic utility of the dioxygenase-derived metabolites of substituted aromatics
The substrate scope and selectivity of toluene dioxygenase overexpressed in E.coli JM109 (pDTG601A) was investigated with series of ortho-halobenzoates and para-substituted arenes. Palladium-catalyzed carbonylation ...