Browsing Ph.D. Biotechnology by Issue Date
Now showing items 1-20 of 26
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The Elucidation of the involvement of endonuclease DNase y in reducing DNA transfection efficiency in mammalian cells
(Brock University, 2005-05-28)Gene therapy is predicated upon efficient gene transfer. While viral vectors are the method of choice for transformation efficiency, the immunogenicity and safety concerns remain problematic. Non-viral vectors, on the ... -
The osmoadaptive response of the wine yeast Saccharomyces cerevisiae K1-V1116 during icewine fermentation
(Brock University, 2008-05-28)The adapted metabolic response of commercial wine yeast under prolonged exposure to concentrated solutes present in Icewine juice is not fully understood. Presently, there is no information regarding the transcriptomic ... -
An exploratory study for the discovery of non-invasive hepatocellular carcinoma biomarkers among high-risk hepatitis C virus infected patients
(Brock University, 2009-01-28)Hepatocellular Carcinoma (HCC) is a major healthcare problem, representing the third most common cause of cancer-related mortality worldwide. Chronic infections with Hepatitis B virus (HBV) and/or Hepatitis C virus (HCV) ... -
Enantiodivergent chemoenzymatic synthesis of codeine
(Brock University, 2009-05-28)The present thesis describes our latest results in the chemistry of morphine alkaloids. An enantiodivergent synthesis of codeine utilizing a cis-cyclohexadiene diol derived from microbial whole cell oxidation of ... -
Unique and unifying themes in the mechanisms regulating the expression of the arabidopsis thaliana PR-1 and Solanum tuberosum PR-10a inducible defense genes
(Brock University, 2010-10-25)Arabidopsis is a model plant used to study disease resistance; Solanum tuberosum or potato is a crop species. Both plants possess inducible defense mechanisms that are deployed upon recognition ... -
Two enantiodivergent syntheses of balanol and the chemoenzymatic synthesis of oseltamivir
(Brock University, 2010-10-25)The present thesis outlines our latest findings on the reactivity of the Burgess reagent with oxiranes. Structural, mechanistic, and computational studies are presented. Included is the development of a ... -
Mechanism of tocopherol transfer by human α-tocopherol transfer protein (α-hTTP)
(Brock University, 2010-10-26)Vitamin E is a well known fat soluble chain breaking antioxidant. It is a general tenn used to describe a family of eight stereoisomers of tocopherols. Selective retention of a-tocopherol in the human circulation ... -
Regulation of Systemic Acquired Resistance through the Interaction of Arabidopsis thaliana Transcription factors TGAI and TGA2 with NPRI
(2012-07-31)Arabidopsis thaliana is an established model plant system for studying plantpathogen interactions. The knowledge garnered from examining the mechanism of induced disease resistance in this model system can be applied to ... -
Icewine fermentation by Saccharomyces cervisiae : fundamental stress responses and comparative fermentation dynamics
(2012-12-13)Icewine is a unique dessert wine fermented from the juice of grapes that have frozen naturally on the vine. Fermentations of 40 Brix (>400g/L soluble solids) juice typically used in Canada to produce Icewine are ... -
ORGANOSILICON BIOTECHNOLOGY: A BIO-INSPIRED APPROACH TO THE HYDROLYSIS OF ALKOXYSILANES and THE LIPASE-CATALYZED SYNTHESIS OF SILOXANE-CONTAINING POLYESTERS AND POLYAMIDES
(Brock University, 2013-02-22)The first part of this thesis studied the capacity of amino acids and enzymes to catalyze the hydrolysis and condensation of tetraethoxysilane and phenyltrimethoxysilane. Selected amino acids were shown to accelerate the ... -
Plant rhizosphere specificity and variability in the insect and plant adhesins, Madl and Mad2, within the genus Metarhizium suggest plant adaptation as an evolutionary force
(2013-04-19)Metarhizium is a soil-inhabiting fungus currently used as a biological control agent against various insect species, and research efforts are typically focused on its ability to kill insects. In section 1, we tested the ... -
STUDYING ABERRANT METHYLATION AND miRNA PROFILING FOR THE DETECTION OF LUNG CANCER BIOMARKERS
(2013-04-19)Lung cancer is a major chronic disease responsible for the highest mortality rate, among other types of cancer, and represents 29% of all deaths in Canada. The clinical diagnosis of lung carcinoma still requires a standard ... -
Prostate Cancer and the Search for Novel Biomarkers
(Brock University, 2013-05-06)The primary objective of this research project was to identify prostate cancer (PCa) -specific biomarkers from urine. This was done using a multi-faceted approach that targeted (1) the genome (DNA); (2) the transcriptome ... -
Functional Characterization of Monoterpenoid Indole Alkaloid (MIA) Biosynthetic Genes in Catharanthus roseus
(Brock University, 2013-08-23)The monoterpenoid indole alkaloids (MIAs) of Madagascar periwinkle (Catharanthus roseus) are known to be among the most important source of natural drugs used in various cancer chemotherapies. MIAs are derived by combining ... -
The γ-tocopherol-like family of N-methyltransferases: A taxonomically clustered gene family encoding enzymes responsible for N-methylation of monoterpene indole alkaloids
(Brock University, 2013-09-19)The plant family Apocynaceae accumulates thousands of monoterpene indole alkaloids (MIAs) which originate, biosynthetically, from the common secoiridoid intermediate, strictosidine, that is formed from the condensation of ... -
The Molecular Consequences of CK2-mediated Phosphorylation of the TGA2 Transcription Factor within Systemic Acquired Resistance of Arabidopsis thaliana
(Brock University, 2014-08-05)During infection, the model plant Arabidopsis thaliana is capable of activating long lasting defence responses both in tissue directly affected by the pathogen and in more distal tissue. Systemic acquired resistance (SAR) ... -
Novel glutathione disulfide transferase function of CC-glutaredoxins involved in disease resistance and flower development
(Brock University, 2014-08-05)Glutaredoxins are oxidoreductases capable of reducing protein disulfide bridges and glutathione mixed disulfides through the process of deglutathionylation and glutathionylation. Lately, redox-mediated modifications of ... -
Towards reverse engineering of Photosystem II: Synergistic Computational and Experimental Approaches
(Brock University, 2014-10-01)ABSTRACT Photosystem II (PSII) of oxygenic photosynthesis has the unique ability to photochemically oxidize water, extracting electrons from water to result in the evolution of oxygen gas while depositing these electrons ... -
Functional genomics of monoterpenoid indole alkaloid biosynthesis in Rauvolfia serpentina
Monoterpenoid indole alkaloids (MIAs) are a large and heterogeneous group of nitrogen-containing specialized metabolites produced by plants belonging to the Apocynaceae, Loganiaceae and Rubiaceae families. Many of these ... -
Trading nitrogen for carbon: Nitrogen and carbon translocation in a plant/fungal (Metarhizium spp.) symbiosis
While nitrogen is critical for all plants, they are unable to utilize organically bound nitrogen in soils. Therefore, the majority of plants obtain useable nitrogen through nitrogen fixing bacteria and the microbial ...