Proton translocation by cytochrome c oxidase reconstituted into proteoliposomes
dc.contributor.author | Shaughnessy, Stephen G. | en_US |
dc.date.accessioned | 2009-07-09T18:42:25Z | |
dc.date.available | 2009-07-09T18:42:25Z | |
dc.date.issued | 1984-07-09T18:42:25Z | |
dc.identifier.uri | http://hdl.handle.net/10464/2019 | |
dc.description.abstract | Cytochrome c oxidase .inserted into proteoliposomes translocates protons with a stoichiometry of approx-, imately 0.4-0.6 H+/e- in the presence of valinomycin plus pottasium. The existance .ofsuchproton translocation is .supportedby experiments with lauryl maltoside which abolished the pulses but~~d not inhibit cyt. c binding .or oxidase turnover. Pulses with K3FeCN6 did not induce acidification further supporting vectorial proton transport by cyt ..aa3 . Upon lowering the ionic strength and pulsing with ferrocytochrome c, H+/eratios increased. This increase is attributed to scaler proton release consequent upon cyt.c-phospholipid binding. Oxygen pulses at low ionic strength however did not exhibit this large scaler increase in H+/e- ratios.A-small increase was observed upon .02 pul'sing at·low ionic strengt.h. This increase was KeN and, ,pcep sensitive and thus possibly due to a redox linked scaler deprotonation. Increases in the H+/e- ratio also occurred ifp~lses ,were performed in the presence of nonactin rather.than valinomycin. The fluorescent pH indicator pyranine was internally trapped inaa3 conta~ning "proteoliposomes. Internal alkalinization, as mon,itored by pyranine fluorescence leads to a of approx.imately 0.35 units, which is proportional to electron flux. This internal alkalinization was also DCCD sensitive, being inhibited by approximately 50%. This 50% inhibition of internal alkalinization supports the existance of vectorial proton transport. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Brock University | en_US |
dc.subject | Protons. | en_US |
dc.subject | Cytochrome c. | en_US |
dc.subject | Cytochrome oxidase. | en_US |
dc.subject | Liposomes. | en_US |
dc.subject | Protease inhibitors. | en_US |
dc.title | Proton translocation by cytochrome c oxidase reconstituted into proteoliposomes | en_US |
dc.type | Electronic Thesis or Dissertation | en |
dc.degree.name | M.Sc. Biological Sciences | en_US |
dc.degree.level | Masters | en_US |
dc.contributor.department | Department of Biological Sciences | en_US |
dc.degree.discipline | Faculty of Mathematics and Science | en_US |
refterms.dateFOA | 2021-07-16T10:58:58Z |