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dc.contributor.authorDhaliwal, Roopan
dc.date.accessioned2023-09-28T18:54:10Z
dc.date.available2023-09-28T18:54:10Z
dc.identifier.urihttp://hdl.handle.net/10464/18137
dc.description.abstractExercise increases browning of white adipose tissue (WAT) however, the mechanisms remain unclear. A factor that is released with exercise, brain derived neurotrophic factor (BDNF), has recently been identified as a possible mediator of WAT browning. Direct treatment of cultured adipocytes with BDNF increases mitochondrial quantity and uncoupling protein 1 (UCP1) content. The purpose of this study was to examine if BDNF has similar effects on adipose tissue browning as endurance training and whether BDNF and endurance training can produce a synergistic effect. Male C57BL6 mice (10-weeks of age) were randomly assigned into one of four groups (n=12/group): 1) sedentary (SED); 2) endurance training (ET) (treadmill running 1 hour/day); 3) BDNF (0.5 mg/kg·bw); or 4) endurance training and BDNF (ET+BDNF) for 5 days/week for 8 weeks. Body mass and food intake were recorded weekly. Following the 8- week intervention, oxygen consumption and energy expenditure were examined via a Promethion metabolic caging system. Mice were anaesthetized, WAT (subcutaneous and inguinal) and brown adipose tissue (BAT; interscapular) depots were collected for histological and protein analysis. BDNF treatment increased serum BDNF, oxygen consumption and energy expenditure to a similar extent as the ET group. ET and BDNF resulted in decreased adipocyte area. Exercise resulted in higher multilocularity, mitochondrial content, UCP1, and higher innervation of iWAT. While BDNF treatment alone resulted in higher PGC1a. Within eWAT, ET showed decreased adipocyte size and higher dynamin related protein 1 (DRP1) protein content suggesting an increased potential in mitochondrial fission. BDNF treatment shows higher innervation of BAT as well as higher mitochondrial protein content among the exercise trained groups with the addition of BDNF. Overall, BDNF and exercise training are able to increase whole-body energy expenditures through markers of adipose tissue browning in a depot-specific manner with some evidence of additive effects.en_US
dc.language.isoengen_US
dc.publisherBrock Universityen_US
dc.subjectadiposeen_US
dc.subjectBDNFen_US
dc.subjectexerciseen_US
dc.subjectbrowningen_US
dc.subjectthermogenesisen_US
dc.titleExamining the effects of BDNF and exercise training on adipose tissue browningen_US
dc.typeElectronic Thesis or Dissertationen_US
dc.degree.nameM.Sc. Applied Health Sciencesen_US
dc.degree.levelMastersen_US
dc.contributor.departmentApplied Health Sciences Programen_US
dc.degree.disciplineFaculty of Applied Health Sciencesen_US


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