Show simple item record

dc.contributor.authorWinter, Karen Judith.en_US
dc.date.accessioned2009-07-09T17:31:38Z
dc.date.available2009-07-09T17:31:38Z
dc.date.issued1985-07-09T17:31:38Z
dc.identifier.urihttp://hdl.handle.net/10464/1768
dc.description.abstractPancreatic deoxyribonuclease preferentially digests active genes during all phases of the cell cycle including mitosis. Recently, a DNAse I-directed in ~ nick translation technique has been used to demonstrate differences in the DNAse I sensitivity of euchromatic and heterochromatic regions of mitotic chromosomes. This ill ~ technique has been used in this study to ask whether facultative heterochromatin of the inactive X chromosome can be distinguished from the active X chromosome in mouse and human tissues. In addition to this, in ~ nick translation has been used to distinguish constitutive heterochromatin in mouse and human mitotic chromosomes. Based on relative levels of DNAse I sensitivity, the inactive X chromosome could not be distinguished from the active X chromosome in either mouse or human tissues but regions of constitutive heterochromatin could be distinguished by their relative DNAse I insensitivity. The use of !D situ nick translation was also applied to tissue sections of 7.5 day mouse embryos to ask whether differing levels of DNAse I sensitivity could be detected between different tissue types. Differences in DNAse I sensitivities were detected in three tissues examined; embryonic ectoderm, an embryo-derived tissue, and two extraembryonic tissues, extraembryonic ectoderm and ectoplacental cone. Embryonic ectoderm and extraembryonic ectoderm nuclei possessed comparable levels of DNAse I sensitivity while ectoplacental cone was significantly less DNAse I sensitive. This suggests that tissue-specific mechanisms such as chromatin structure may be involved in the regulation of gene activity in certain tissue types. This may also shed some light on possible tissue specific mechanisms regulating X chromosome activity in the developing mouse embryo.en_US
dc.language.isoengen_US
dc.publisherBrock Universityen_US
dc.subjectChromatic.en_US
dc.subjectMice--Physiology.en_US
dc.subjectTissues--Analysis.en_US
dc.titleAssessment of chromatin activity in mouse and human tissuesen_US
dc.typeElectronic Thesis or Dissertationen_US
dc.degree.nameM.Sc. Biological Sciencesen_US
dc.degree.levelMastersen_US
dc.contributor.departmentDepartment of Biological Sciencesen_US
dc.degree.disciplineFaculty of Mathematics and Scienceen_US
refterms.dateFOA2021-08-07T02:05:14Z


Files in this item

Thumbnail
Name:
Brock_Winter_Karen_1985.pdf
Size:
10.55Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record