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dc.contributor.authorAlva, Ricardo
dc.contributor.authorGardner, Georgina L.
dc.contributor.authorLiang, Ping
dc.contributor.authorStuart, Jeffrey A.
dc.date.accessioned2022-10-17T13:31:29Z
dc.date.available2022-10-17T13:31:29Z
dc.date.issued2022
dc.identifier.citationCells. 2022 Oct 4;11(19):3123en_US
dc.identifier.issn2073-4409
dc.identifier.urihttp://hdl.handle.net/10464/16871
dc.description.abstractMost conventional incubators used in cell culture do not regulate O2 levels, making the headspace O2 concentration ~18%. In contrast, most human tissues are exposed to 2-6% O2 (physioxia) in vivo. Accumulating evidence has shown that such hyperoxic conditions in standard cell culture practices affect a variety of biological processes. In this review, we discuss how supraphysiological O2 levels affect reactive oxygen species (ROS) metabolism and redox homeostasis, gene expression, replicative lifespan, cellular respiration, and mitochondrial dynamics. Furthermore, we present evidence demonstrating how hyperoxic cell culture conditions fail to recapitulate the physiological and pathological behavior of tissues in vivo, including cases of how O2 alters the cellular response to drugs, hormones, and toxicants. We conclude that maintaining physioxia in cell culture is imperative in order to better replicate in vivo-like tissue physiology and pathology, and to avoid artifacts in research involving cell culture.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.subjectROSen_US
dc.subjectDrug responseen_US
dc.subjectGene expressionen_US
dc.subjectHyperoxiaen_US
dc.subjectMetabolismen_US
dc.subjectMitochondrial dynamicsen_US
dc.subjectOxidative stressen_US
dc.subjectOxygenen_US
dc.subjectPhysioxiaen_US
dc.subjectSenescenceen_US
dc.titleSupraphysiological Oxygen Levels in Mammalian Cell Culture: Current State and Future Perspectivesen_US
dc.typeArticleen_US
dc.identifier.doi10.3390/cells11193123
refterms.dateFOA2022-10-17T13:31:29Z


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