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dc.contributor.authorBott, Kirsten
dc.date.accessioned2022-01-31T19:18:55Z
dc.date.available2022-01-31T19:18:55Z
dc.identifier.urihttp://hdl.handle.net/10464/15602
dc.description.abstractChronic low-grade inflammation has been identified as a potential contributor to the pathophysiology of osteoporosis. A key mediator may be lipopolysaccharide (LPS) released from gram-negative bacteria in the gut that can enter circulation stimulating an inflammatory response and upregulate bone resorption. Since rodent models mimic the loss of bone mineral density (BMD) and structure that occurs in humans, rodents offer an accelerated model for studying these inflammation-mediated changes. Therefore, the objective of this thesis was to characterize a rodent model of LPS-induced bone loss using repeated in vivo μCT scans to establish a time course effect of LPS longitudinally and for this purpose three studies were conducted. Study 1 & 2 were run simultaneously using the same control mice. Study 1 demonstrated that repeated irradiation had a negative impact on trabecular bone in both male and female CD-1 mice, while cortical bone was only negatively impacted in the females. In study 2, continuous delivery of exogenous LPS via osmotic pumps for 12 weeks elevated serum LPS in both male and female CD-1 mice but did not alter trabecular or cortical bone structure or BMD at any of the scanning timepoints. Results from Study 2 may in part have been influenced by the effects of repeated irradiation from the in vivo μCT scans at 4-week intervals for a total of 4 scans analyzed in Study 1. In study 3, a systematic review was conducted to better characterize a model of LPS induced bone loss and identify factors that may impact the effects of LPS on bone outcomes in rodent models. Regardless of study duration, exogenous LPS negatively impacted trabecular bone structure and BMD but not cortical bone structure, due to an upregulation in bone resorption. Together these data suggest that exogenous LPS can induce alterations in bone structure and BMD in rodent models, however a clearly defined model of exogenous LPS induced bone loss has yet to be fully characterized.en_US
dc.language.isoengen_US
dc.publisherBrock Universityen_US
dc.subjecttrabecular boneen_US
dc.subjectcortical boneen_US
dc.subjectmicro-computed tomographyen_US
dc.subjectlipopolysaccharideen_US
dc.subjectrodent modelen_US
dc.titleEffects of Exogenous Lipopolysaccharide Exposure on Bone Outcomes in Rodent Modelsen_US
dc.typeElectronic Thesis or Dissertationen_US
dc.degree.namePh.D. Applied Health Sciencesen_US
dc.degree.levelDoctoralen_US
dc.contributor.departmentApplied Health Sciences Programen_US
dc.degree.disciplineFaculty of Applied Health Sciencesen_US


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