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    A ‘green’ approach to fixing polyacrylamide gels

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    Author
    Carbonara, Katrina
    Coorssen, Jens R.
    Keyword
    Fixation
    Proteomes
    Gel electrophoresis
    Proteomic
    Eco-friendly
    Staining and labeling- methods
    Water pollutants, chemical
    Brain
    Animals
    Occupational health
    Electrophoresis, gel, two-dimensional - methods
    Rats
    Proteins- analysis
    Acrylic resins- chemistry
    Electrophoresis, polyacrylamide gels- methods
    Len plants- chemistrt
    Index Medicus
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    URI
    http://hdl.handle.net/10464/15092
    Abstract
    Handling chemicals that require specific safety precautions and protections generates the need for hazardous waste removal and transportation costs. With the growing effort to reduce both cost per analysis and the environmental footprint of research, we report an effective alternative to the widely used methanol/acetic acid gel fixation solution. 1.0 M citric acid dissolved in 5% acetic acid (C3A) provides comparable results following both SDS-PAGE and two-dimensional gel electrophoresis, while also eliminating waste removal costs. •Citric acid dissolved in acetic acid replaces current gel fixative solution.•Cost per analysis is reduced and hazardous waste removal costs eliminated.•Environmental footprint of research is reduced.
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.ab.2020.113853
    Scopus Count
    Collections
    Chemistry

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