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dc.contributor.authorWang, Guan
dc.date.accessioned2021-05-10T19:45:47Z
dc.date.available2021-05-10T19:45:47Z
dc.identifier.urihttp://hdl.handle.net/10464/15074
dc.description.abstractIn the last decades, various DNA hybridization probes have been developed that attempt to conquer the challenge of single-nucleotide-variants (SNVs) detection. Even though a powerful toolbox including the toehold-exchange reaction, the dynamic ‘sink’ design, and the polymerase chain reaction (PCR) has been built, it still faces practical problems. For example, the natural DNA is usually in double-stranded form whereas most hybridization probes aim for single-stranded targets; the concentration of extracted DNA samples is totally unknown thus may lay outside the optimal design of probes/primers. To achieve ultra-high sensitivity and specificity, expensive and sophisticated machines such as digital droplet PCR and next-generation-sequencing may be inapplicable in rural areas. Therefore, the quantitative PCR method is still the gold standard for clinical tests. Thus motivated, my PhD career was mainly focused on the fundamental understanding of the challenges in SNVs discrimination and developing robust, versatile, and user-friendly probes/strategies. In this thesis, Chapter 1 provides a general introduction of dynamic DNA nanotechnology and its representative applications in discriminating SNVs. Chapter 2 to 4 describe three completed projects that aim to understand the thermodynamic and kinetic properties of strand displacement reactions and to circumvent the challenges of discriminating SNVs through finely tuned probes/assays.en_US
dc.language.isoengen_US
dc.publisherBrock Universityen_US
dc.subjectDNA Nanotechnologyen_US
dc.subjectSingle nucleotide varianten_US
dc.subjectDNA hybridization probeen_US
dc.titleDynamic DNA Nanotechnology for Probing Single Nucleotide Variants and DNA Modificationsen_US
dc.typeElectronic Thesis or Dissertationen_US
dc.degree.namePh.D. Chemistryen_US
dc.degree.levelDoctoralen_US
dc.contributor.departmentDepartment of Chemistryen_US
dc.degree.disciplineFaculty of Mathematics and Scienceen_US
refterms.dateFOA2021-08-18T01:30:05Z


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