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dc.contributor.authorMoonjely, Soumya
dc.contributor.authorZhang, Xing
dc.contributor.authorFang, Weiguo
dc.contributor.authorBidochka, Michael J
dc.date.accessioned2020-05-08T17:16:41Z
dc.date.available2020-05-08T17:16:41Z
dc.date.issued2019
dc.identifier.citationPLoS ONE, 01 January 2019, Vol.14(10), p.e0223718en_US
dc.identifier.issn1932-6203
dc.identifier.issn10.1371/journal.pone.0223718
dc.identifier.urihttp://hdl.handle.net/10464/14824
dc.description.abstractThe endophytic insect pathogenic fungi (EIPF) Metarhizium promotes plant growth through symbiotic association and the transfer of insect-derived nitrogen. However, little is known about the genes involved in this association and the transfer of nitrogen. In this study, we assessed the involvement of six Metarhizium robertsii genes in endophytic, rhizoplane and rhizospheric colonization with barley roots. Two ammonium permeases (MepC and Mep2) and a urease, were selected since homologous genes in arbuscular mycorrhizal fungi were reported to play a pivotal role in nitrogen mobilization during plant root colonization. Three other genes were selected on the basis on RNA-Seq data that showed high expression levels on bean roots, and these encoded a hydrophobin (Hyd3), a subtilisin-like serine protease (Pr1A) and a hypothetical protein. The root colonization assays revealed that the deletion of urease, hydrophobin, subtilisin-like serine protease and hypothetical protein genes had no impact on endophytic, rhizoplane and rhizospheric colonization at 10 or 20 days. However, the deletion of MepC resulted in significantly increased rhizoplane colonization at 10 days whereas ΔMep2 showed increased rhizoplane colonization at 20 days. In addition, the nitrogen transporter mutants also showed significantly higher 15N incorporation of insect derived nitrogen in barley leaves in the presence of nutrients. Insect pathogenesis assay revealed that disruption of MepC, Mep2, urease did not reduce virulence toward insects. The enhanced rhizoplane colonization of ΔMep2 and ΔMepC and insect derived nitrogen transfer to plant hosts suggests the role of MepC and Mep2 in Metarhizium-plant symbiosis.en_US
dc.description.sponsorshipBrock University Library Open Access Publishing Funden_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.subjectSciences (General)en_US
dc.titleMetarhizium robertsii ammonium permeases (MepC and Mep2) contribute to rhizoplane colonization and modulates the transfer of insect derived nitrogen to plantsen_US
dc.typeArticleen_US
refterms.dateFOA2021-08-18T01:46:58Z


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