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dc.contributor.authorWaller, Alison S.
dc.contributor.authorBehie, Scott W.
dc.contributor.authorBidochka, Michael J.
dc.contributor.authorBarelli, Larissa
dc.date.accessioned2020-05-05T19:33:40Z
dc.date.available2020-05-05T19:33:40Z
dc.date.issued2020-01
dc.identifier.citationPLoS ONE, 01 January 2020, Vol.15(4), p.e0231150en_US
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10464/14816
dc.description.abstractThe microbial community in the plant rhizosphere is vital to plant productivity and disease resistance. Alterations in the composition and diversity of species within this community could be detrimental if microbes suppressing the activity of pathogens are removed. Species of the insect-pathogenic fungus, Metarhizium, commonly employed as biological control agents against crop pests, have recently been identified as plant root colonizers and provide a variety of benefits (e.g. growth promotion, drought resistance, nitrogen acquisition). However, the impact of Metarhizium amendment on the rhizosphere microbiome has yet to be elucidated. Using Illumina sequencing, we examined the community profiles (bacteria and fungi) of common bean (Phaseolus vulgaris) rhizosphere (loose soil and plant root) after amendment with M. robertsii conidia, in the presence and absence of an insect host. Although alpha diversity was not significantly affected overall, there were numerous examples of plant growth-promoting organisms that significantly increased with Metarhizium amendment (Bradyrhizobium, Flavobacterium, Chaetomium, Trichoderma). Specifically, the abundance of Bradyrhizobium, a group of nitrogen-fixing bacteria, was confirmed to be increased using a qPCR assay with genus-specific primers. In addition, the ability of the microbiome to suppress the activity of a known bean root pathogen was assessed. The development of disease symptoms after application with Fusarium solani f. sp. phaseoli was visible in the hypocotyl and upper root of plants grown in sterilized soil but was suppressed during growth in microbiome soil and soil treated with M. robertsii. Successful amendment of agricultural soils with biocontrol agents such as Metarhizium necessitates a comprehensive understanding of the effects on the diversity of the rhizosphere microbiome. Such research is fundamentally important towards sustainable agricultural practices to improve overall plant health and productivity.en_US
dc.description.sponsorshipBrock University Library Open Access Publishing Funden_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.subjectMetarhiziumen_US
dc.subjectBeansen_US
dc.subjectMicrobiomeen_US
dc.subjectLarvaeen_US
dc.subjectFungien_US
dc.subjectFusariumen_US
dc.subjectPlant fungal pathogensen_US
dc.subjectRhizosphereen_US
dc.titlePlant microbiome analysis after Metarhizium amendment reveals increases in abundance of plant growth-promoting organisms and maintenance of disease-suppressive soilen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0231150


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