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dc.contributor.authorHameed, Muhammad Salman
dc.contributor.authorWang, Zhengbing
dc.contributor.authorVasseur, Liette
dc.contributor.authorYang, Guang
dc.date.accessioned2020-04-24T17:35:34Z
dc.date.available2020-04-24T17:35:34Z
dc.date.issued2018-04-01
dc.identifier.citationInternational Journal of Molecular Sciences, 01 April 2018, Vol.19(4), p.1249en_US
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/10464/14806
dc.description.abstractArgonaute (Ago) protein family plays a key role in the RNA interference (RNAi) process in different insects including Lepidopteran. However, the role of Ago proteins in the RNAi pathway of Plutella xylostella is still unknown. We cloned an Argonaute3 gene in P. xylostella ( PxAgo3 ) with the complete coding sequence of 2832 bp. The encoded protein had 935 amino acids with an expected molecular weight of 108.9 kDa and an isoelectric point of 9.29. It contained a PAZ (PIWI/Argonaute/Zwile) domain and PIWI (P-element-induced whimpy testes) domain. PxAgo3 was classified into the Piwi subfamily of Ago proteins with a high similarity of 93.0% with Bombyx mori Ago3 (BmAgo3). The suppression of PxAgo3 by dsPxAgo3 was observed 3 h after treatment and was maintained until 24 h. Knockdown of PxAgo3 decreased the suppression level of PxActin by dsPxActin in P. xylostella cells, while overexpression of PxAgo3 increased the RNAi efficiency. Our results suggest that PxAgo3 play a key role in the double stranded RNA (dsRNA)-regulated RNAi pathway in P. xylostella .en_US
dc.language.isoen_USen_US
dc.publisherMDPIen_US
dc.subjectLepidopteran Pesten_US
dc.subjectPxago3en_US
dc.subjectPhylogenetic Treeen_US
dc.subjectDsrnaen_US
dc.subjectRnai Processen_US
dc.subjectBiologyen_US
dc.titleMolecular Characterization and the Function of Argonaute3 in RNAi Pathway of Plutella xylostellaen_US
dc.typeArticleen_US
dc.identifier.doi10.3390/ijms19041249
refterms.dateFOA2021-08-18T01:40:57Z


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