Host Plant-Derived miRNAs Potentially Modulate the Development of a Cosmopolitan Insect Pest, Plutella xylostella
Author
Zhang, Ling-LingJIng, Xiao-Dong
Chen, Wei
Wang, Yue
Lin, Jun-Han
Zheng, Ling
Dong, Yu-Hong
Zhou, Li
Li, Fei-Fei
Yang, Fei-Ying
Peng, Lu
Vasseur, Liette
He, Wei-Yi
You, Min-Sheng
Keyword
Diamondback mothPlant-derived microrna
Cross-kingdom
Bjhsp1
Ppo2
Development
Anatomy & physiology
Metadata
Show full item recordAbstract
Plant microRNAs (miRNAs) have recently been reported to be involved in the cross-kingdom regulation of specific cellular and physiological processes in animals. However, little of this phenomenon is known for the communication between host plant and insect herbivore. In this study, the plant-derived miRNAs in the hemolymph of a cruciferous specialist were identified by small RNAs sequencing. A total of 39 miRNAs with typical characteristics of plant miRNAs were detected, of which 24 had read counts ≥ 2 in each library. Three plant-derived miRNAs with the highest read counts were validated, and all of them were predicted to target the hemocyanin domains-containing genes of . The luciferase assays in the S2 cell demonstrated that miR159a and novel-7703-5p could target and respectively, possibly in an incomplete complementary pairing mode. We further found that treatment with agomir-7703-5p significantly influenced the pupal development and egg-hatching rate when reared on the artificial...ae974a485f413a2113503eed53cd6c53
10.3390/biom9100602