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dc.contributor.authorDong, Tianyu
dc.date.accessioned2019-10-24T19:00:16Z
dc.date.available2019-10-24T19:00:16Z
dc.identifier.urihttp://hdl.handle.net/10464/14549
dc.description.abstractThis thesis is focused on the development of field-deployable nucleic acid testing platforms to allowed rapid detection and quantification of nucleic acids. Two distinct platforms suitable for nucleic acid testing in resource-limited settings were developed. First, a paper-based diagnostic device was developed. The principle of this paper-based device was based on the unique interfacial interaction of DNA and the DNA intercalating dye with cellulose on chromatographic paper. Second, a colorimetric reader was developed. The principle of the reader was based on measuring the absorbance change of a chromogenic substrate which is triggered by DNA and DNA intercalating dyes under light illumination. The performance of both devices was tested using synthetic DNA, nucleic acid amplicons, and actual parasites nucleic acid samples collected from school-age children in rural areas of Honduras.en_US
dc.language.isoengen_US
dc.publisherBrock Universityen_US
dc.subjectNucleic acid testingen_US
dc.subjectPoint-of-careen_US
dc.subjectPaper based deviceen_US
dc.subjectSoil-transmitted helminthen_US
dc.titleDevelopment of Field-deployable Nucleic Acid Testing Platformsen_US
dc.typeElectronic Thesis or Dissertationen_US
dc.degree.nameM.Sc. Chemistryen_US
dc.degree.levelMastersen_US
dc.contributor.departmentDepartment of Chemistryen_US
dc.degree.disciplineFaculty of Mathematics and Scienceen_US


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