Show simple item record

dc.contributor.authorJones, Graham
dc.date.accessioned2019-06-14T12:39:29Z
dc.date.available2019-06-14T12:39:29Z
dc.identifier.urihttp://hdl.handle.net/10464/14182
dc.description.abstractCatharanthus roseus, a member of the Apocynaceae family, has been studied extensively for its valuable chemotherapeutic monoterpenoid indole alkaloids (MIAs). Ethyl methanesuphonate (EMS) mutagenesis is a screening tool that has been used to look for altered MIA profiles in hope of discovering mutations of crucial MIA biosynthetic genes. Without a high-throughput mutation detection screen for C. roseus sequencing data, a range of techniques must be used to discover the EMS-induced changes within the plant. Bioinformatic and morphological analysis revealed the likely alterations leading to unique MIA profiles in two C. roseus EMS mutants: the high-ajmalicine accumulating line M2-0754 and the low-MIA accumulating line M2-1582. Expression of geissoschizine synthase (GS) was downregulated almost seven-fold in the leaves of M2-0754, leading to the accumulation of an alternate pathway MIA from the labile intermediate. The low-MIA profile and increased auxin sensitivity of M2-1582 is likely due to the expression of a dysfunctional auxin influx transport protein homologue.en_US
dc.language.isoengen_US
dc.publisherBrock Universityen_US
dc.subjectCatharanthusen_US
dc.subjectroseusen_US
dc.subjectmutagenesisen_US
dc.subjectbioinformaticsen_US
dc.subjectmorphologyen_US
dc.subjectEMSen_US
dc.subjectplanten_US
dc.titleBioinformatic and morphological characterization of Catharanthus roseus mutantsen_US
dc.typeElectronic Thesis or Dissertationen_US
dc.degree.nameM.Sc. Biotechnologyen_US
dc.degree.levelMastersen_US
dc.contributor.departmentCentre for Biotechnologyen_US
dc.degree.disciplineFaculty of Mathematics and Scienceen_US
refterms.dateFOA2021-08-15T01:41:46Z


Files in this item

Thumbnail
Name:
Brock_Jones_Graham_2019
Size:
3.219Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record