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dc.contributor.authorWalker, Sarah E
dc.contributor.authorSpencer, Gaynor E.
dc.contributor.authorNecakov, Aleksander
dc.contributor.authorCarlone, Robert L.
dc.date.accessioned2018-12-04T17:38:15Z
dc.date.available2018-12-04T17:38:15Z
dc.date.issued2018-09-13
dc.identifier.citationInt. J. Mol. Sci. 2018, 19, 2741; doi:10.3390/ijms19092741en_US
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/10464/13825
dc.description.abstractRetinoic acid (RA) is the biologically active metabolite of vitamin A and has become a well-established factor that induces neurite outgrowth and regeneration in both vertebrates and invertebrates. However, the underlying regulatory mechanisms that may mediate RA-induced neurite sprouting remain unclear. In the past decade, microRNAs have emerged as important regulators of nervous system development and regeneration, and have been shown to contribute to processes such as neurite sprouting. However, few studies have demonstrated the role of miRNAs in RA-induced neurite sprouting. By miRNA sequencing analysis, we identify 482 miRNAs in the regenerating central nervous system (CNS) of the mollusc Lymnaea stagnalis, 219 of which represent potentially novel miRNAs. Of the remaining conserved miRNAs, 38 show a statistically significant up- or downregulation in regenerating CNS as a result of RA treatment. We further characterized the expression of one neuronally-enriched miRNA upregulated by RA, miR-124. We demonstrate, for the first time, that miR-124 is expressed within the cell bodies and neurites of regenerating motorneurons. Moreover, we identify miR-124 expression within the growth cones of cultured ciliary motorneurons (pedal A), whereas expression in the growth cones of another class of respiratory motorneurons (right parietal A) was absent in vitro. These findings support our hypothesis that miRNAs are important regulators of retinoic acid-induced neuronal outgrowth and regeneration in regeneration-competent species.en_US
dc.description.sponsorshipBrock University Library Open Access Publishing Funden_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.subjectretinoic aciden_US
dc.subjectmicroRNAen_US
dc.subjectRNA sequencingen_US
dc.subjectneuronal regenerationen_US
dc.subjectgrowth coneen_US
dc.subjectLymnaeaen_US
dc.titleIdentification and Characterization of microRNAs during Retinoic Acid-Induced Regeneration of a Molluscan Central Nervous Systemen_US
dc.typeArticleen_US
dc.identifier.doi10.3390/ijms19092741
refterms.dateFOA2021-08-12T01:58:10Z


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