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dc.contributor.authorNash, Leslie Alanna
dc.date.accessioned2014-09-02T19:48:40Z
dc.date.issued2014-09-02
dc.identifier.urihttp://hdl.handle.net/10464/5644
dc.description.abstractSome cross-sectional and prospective studies have demonstrated a positive correlation between habitual tea consumption and bone mineral density in post-menopausal women. Rooibos tea contains no caffeine and is a rich source of flavonoids such as rutin, orientin, hyperoside and luteolin. These flavonoids have similar structures to estradiol, and therefore may act as estrogen mimics to promote favourable outcomes in bone. The overall objective of this research was to identify flavonoids that could enhance mineral content in human osteoblast Saos2 cells. Mineral was quantified by alizarin red staining and characterized by quantifying alkaline phosphatase (ALP) activity, cell mitochondria activity and toxicity, in addition to changes in regulatory markers of osteoblastic activity. Rutin (≥50μM), hyperoside (≥5.0μM), orientin (0.1μM-1.0μM, 15μM-100μM) and luteolin (5.0μM) enhanced mineral content. This was in part due to elevated ALP and mitochondrial activity, and lower toxicity, pro-inflammatory cytokines, and Wnt inhibitors.en_US
dc.language.isoengen_US
dc.publisherBrock Universityen_US
dc.subjectalkaline phosphataseen_US
dc.subjectflavonoidsen_US
dc.subjectmineralen_US
dc.subjectosteoblasten_US
dc.subjectrooibos teaen_US
dc.titleRooibos tea flavonoids increase mineral content in human osteoblast-like cellsen_US
dc.typeElectronic Thesis or Dissertationen_US
dc.degree.nameM.Sc. Applied Health Sciencesen_US
dc.degree.levelMastersen_US
dc.contributor.departmentApplied Health Sciences Programen_US
dc.degree.disciplineFaculty of Applied Health Sciencesen_US
dc.embargo.lift2015-08-28T19:48:40Z
dc.embargo.terms12 Monthsen_US


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