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Role of Exopolysaccharides and Monosaccharides in Erwinia amylovora Resistance to Bacteriophages

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Show simple item record Sjaarda, David 2012-08-09T13:16:47Z 2012-08-09T13:16:47Z 2012-08-09
dc.description.abstract Fire blight is a disease caused by the phytopathogenic bacterium Erwinia amylovora, an economically important pathogen in the commercial production of apples and pears. Bacteriophages have been proposed as a commercial biopesticide to relieve the pressures on apple and pear production and provide alternatives to existing biological control options. This work reports on the investigation of host resistance in the development of a phage biopesticide. Exopolysaccharide (EPS) deficient bacterial mutants were generated through recombineering to investigate the role of EPS in bacteriophage adsorption and infection. The mutants that were deficient in amylovoran production were avirulent and resistant to infection by phages of the Podoviridae and some of the Siphoviridae family. Levan deficient bacterial mutants resulted in reduced phage titers in some phages from the Myoviridae family. Exopolysaccharide mimetic monosaccharides were used to demonstrate that levan and amylovoran play an important role in phage attack of E. amylovora. en_US
dc.language.iso eng en_US
dc.publisher Brock University en_US
dc.subject Bacteriophage en_US
dc.subject Erwinia amylovora en_US
dc.subject Fire Blight en_US
dc.subject Bacterial transformation en_US
dc.subject EPS en_US
dc.subject Exopolysaccharides en_US
dc.title Role of Exopolysaccharides and Monosaccharides in Erwinia amylovora Resistance to Bacteriophages en_US
dc.type Electronic Thesis or Dissertation en_US M.Sc. Biological Sciences en_US Masters en_US
dc.contributor.department Department of Biological Sciences en_US Faculty of Mathematics and Science en_US

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