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Role of Exopolysaccharides and Monosaccharides in Erwinia amylovora Resistance to Bacteriophages

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dc.contributor.author Sjaarda, David
dc.date.accessioned 2012-08-09T13:16:47Z
dc.date.available 2012-08-09T13:16:47Z
dc.date.issued 2012-08-09
dc.identifier.uri http://hdl.handle.net/10464/4086
dc.description.abstract Fire blight is a disease caused by the phytopathogenic bacterium Erwinia amylovora, an economically important pathogen in the commercial production of apples and pears. Bacteriophages have been proposed as a commercial biopesticide to relieve the pressures on apple and pear production and provide alternatives to existing biological control options. This work reports on the investigation of host resistance in the development of a phage biopesticide. Exopolysaccharide (EPS) deficient bacterial mutants were generated through recombineering to investigate the role of EPS in bacteriophage adsorption and infection. The mutants that were deficient in amylovoran production were avirulent and resistant to infection by phages of the Podoviridae and some of the Siphoviridae family. Levan deficient bacterial mutants resulted in reduced phage titers in some phages from the Myoviridae family. Exopolysaccharide mimetic monosaccharides were used to demonstrate that levan and amylovoran play an important role in phage attack of E. amylovora. en_US
dc.language.iso eng en_US
dc.publisher Brock University en_US
dc.subject Bacteriophage en_US
dc.subject Erwinia amylovora en_US
dc.subject Fire Blight en_US
dc.subject Bacterial transformation en_US
dc.subject EPS en_US
dc.subject Exopolysaccharides en_US
dc.title Role of Exopolysaccharides and Monosaccharides in Erwinia amylovora Resistance to Bacteriophages en_US
dc.type Electronic Thesis or Dissertation en_US
dc.degree.name M.Sc. Biological Sciences en_US
dc.degree.level Masters en_US
dc.contributor.department Department of Biological Sciences en_US
dc.degree.discipline Faculty of Mathematics and Science en_US


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