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Protein Turnover in Rat Skeletal Muscle During In Vitro Hypo-Osmotic Stress

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dc.contributor.author Darling, Ryan
dc.date.accessioned 2012-01-16T13:53:30Z
dc.date.available 2012-01-16T13:53:30Z
dc.date.issued 2012-01-16
dc.identifier.uri http://hdl.handle.net/10464/3894
dc.description.abstract Hypo-osmolality influences tissue metabolism, but research on protein turnover in skeletal muscle is limited. The purpose of this investigation was to examine the effects of hypo-osmotic stress on protein turnover in rat skeletal muscle. We hypothesized increased protein synthesis and reduced degradation following hypo-osmotic exposure. EDL muscles (n=8/group) were incubated in iso-osmotic (290 Osm/kg) or hypo-osmotic (190 Osm/kg) modified medium 199 (95% O2, 5% CO2, pH 7.4, 30±2 °C) for 60 min, followed by 75 min incubations with L-U[14C]phenylalanine or cycloheximide to determine protein synthesis and degradation. Immunoblotting was performed to assess signalling pathways involved. Phenylalanine uptake and incorporation were increased by 199% and 169% respectively in HYPO from ISO (p < 0.05). This was supported by elevated phosphorylation of mTOR Ser2448 (+12.5%) and increased Thr389 phosphorylation on p70s6 kinase (+23.6%) (p < 0.05). Hypo-osmotic stress increased protein synthesis and potentially amino acid uptake. Future studies should examine the upstream mechanisms involved. en_US
dc.language.iso eng en_US
dc.publisher Brock University en_US
dc.subject cell swelling en_US
dc.subject protein turnover en_US
dc.subject skeletal muscle en_US
dc.subject metabolism en_US
dc.subject hydration en_US
dc.title Protein Turnover in Rat Skeletal Muscle During In Vitro Hypo-Osmotic Stress en_US
dc.type Electronic Thesis or Dissertation en_US
dc.degree.name M.Sc. Applied Health Sciences en_US
dc.degree.level Masters en_US
dc.contributor.department Applied Health Sciences Program en_US
dc.degree.discipline Faculty of Applied Health Sciences en_US


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