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A possible role for chemokine signalling through CXCR4 as a downstream effector of retinoic acid signalling in the regenerating tail and spinal cord of Notophthalmus viridescens

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dc.contributor.author Ricci, Anthony
dc.date.accessioned 2011-05-17T17:37:22Z
dc.date.available 2011-05-17T17:37:22Z
dc.date.issued 2011-05-17
dc.identifier.uri http://hdl.handle.net/10464/3370
dc.description.abstract The newt, Notopthalmus viridescens is one of the few tet rapod vertebrates capable of extensive regeneration of the central nervous system, however, the factors involved in this process are still unknown. Chemokine signalling through the receptor CXCR4, has been found to be involved in the development of the central nervous system of mammals and more recently in epimorphic fin regeneration in zebrafish. We have hypothesized that the CXCR4 signalling pathway is involved in spinal cord and tail regeneration in the adul t newt , possibly as a downstream target of retinoic acid signalling. We found that CXCR4 mRNA expression was observed in the brain, spinal cord, heart, gut, liver and regenerating tail blastemas. CXCR4 expression increased over the f i rst 12 days of tail regeneration and returned to basal expression levels at day 21 of regeneration. Inhibition of CXCR4 wi th AMD3100, a specific receptor antagonist, led to a decrease in CXCR4 mRNA in the regenerating tail 14 days post amputation. Histological analysis suggests a delay in the early stages of tail and spinal cord regeneration. Spinal cord explants t reated wi th CXCL12, the ligand to CXCR4, displayed enhanced neurite outgrowth in vitro. Explants t reated wi th AMD3100 abolished any retinoic acid enhanced neurite outgrowth effects suggesting a link between these signalling pathways. en_US
dc.language.iso eng en_US
dc.publisher Brock University en_US
dc.subject Notophthalmus viridescens -- Anatomy en_US
dc.subject Regeneration (Biology) en_US
dc.subject Nervous system -- Regeneration en_US
dc.title A possible role for chemokine signalling through CXCR4 as a downstream effector of retinoic acid signalling in the regenerating tail and spinal cord of Notophthalmus viridescens en_US
dc.type Electronic Thesis or Dissertation en_US
dc.degree.name M.Sc. Biological Sciences en_US
dc.degree.level Masters en_US
dc.contributor.department Department of Biological Sciences en_US
dc.degree.discipline Faculty of Mathematics and Science en_US


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