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The Structural and Functional Changes of Blood Vessels during Aging

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dc.contributor.author Ratsep, Matthew T
dc.date.accessioned 2011-02-28T19:30:20Z
dc.date.available 2011-02-28T19:30:20Z
dc.date.issued 2011-02-28
dc.identifier.uri http://hdl.handle.net/10464/3143
dc.description.abstract The vascular adventitia is recognized as a dynamic mediator of vascular structure and function, yet its role in aging is not understood. The purpose of this thesis was to examine the age-related changes of the vascular adventitia and determine the underlying mediators responsible. Male Sprague-Dawley rats were aged to 15, 30, 50 and 80 weeks before being anesthetised and euthanized by exsanguination. Thoracic aortas, mesenteric and pudental arteries were isolated, formalin fixed, and embedded in paraffin then sectioned at 5μm. Vessels were examined by microscopy and protein expression was determined by indirect immunofluorescence. The thickness of the adventitia increased dramatically with age. Immunofluorescence revealed a robust expression of endothelin system proteins in the adventitia. Additionally, extracellular matrix proteins collagen and fibronectin, and the proliferation marker Ki67 showed strong adventitial origin. The changes observed in the vascular adventitia with aging clearly demonstrate an important role in the process of vascular aging. en_US
dc.language.iso eng en_US
dc.publisher Brock University en_US
dc.subject Adventitia en_US
dc.subject Endothelin en_US
dc.subject Aging en_US
dc.subject Hypertension en_US
dc.subject Fibroblast en_US
dc.title The Structural and Functional Changes of Blood Vessels during Aging en_US
dc.type Electronic Thesis or Dissertation en_US
dc.degree.name M.Sc. Applied Health Sciences en_US
dc.degree.level Masters en_US
dc.contributor.department Applied Health Sciences Program en_US
dc.degree.discipline Faculty of Applied Health Sciences en_US


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