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Fungal biotransformation of morphine alkaloids

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dc.contributor.author Chaudhary, Vigi. en_US
dc.date.accessioned 2010-02-16T15:45:55Z
dc.date.available 2010-02-16T15:45:55Z
dc.date.issued 2009-02-16T15:45:55Z
dc.identifier.uri http://hdl.handle.net/10464/2907
dc.description.abstract The purpose of the study was to determine the ability of certain fungi to biotransform morphine alkaloids into medicinally relevant intermediates. Fungal strains screened for their ability to affect biotransformation of morphine alkaloids include Cunninghamella echinulata, Helicostylum pirijorme, Pycnoporus sanguinea, Pycnoporus cinnabarina, Curvularia lunata and Sporotrichum sulfurescens. The research demonstrated that Cunninghamella echinulata N-demethylated thebaine, hydrocodone, codeine, oripavine and oxycodone into corresponding nor-compounds in varying yields. The study further focused on the characterization of the enzyme responsible for the biotransformation of thebaine into northebaine by Cunninghamella echinulata. The study clearly showed that incubation of the fungal culture with thebaine over a period of 48 hours was required to activate the biotransformation process. The biotransformation studies with [14C] labeled thebaine showed that Ndemethylation by Cunningham ella echinulata does not involve O-demethylation followed by methyl group transfer as suggested in previous studies. en_US
dc.language.iso eng en_US
dc.publisher Brock University en_US
dc.subject Morphine--Synthesis. en_US
dc.subject Microbial biotechnology. en_US
dc.subject Enzymes--Biotechnology. en_US
dc.subject Biotransformation (Metabolism) en_US
dc.title Fungal biotransformation of morphine alkaloids en_US
dc.type Electronic Thesis or Dissertation en_US
dc.degree.name M.Sc. Biological Sciences en_US
dc.degree.level Masters en_US
dc.contributor.department Department of Biological Sciences en_US
dc.degree.discipline Faculty of Mathematics and Science en_US


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