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dc.contributor.authorWest, Ryan.en_US
dc.date.accessioned2010-01-28T15:55:28Z
dc.date.available2010-01-28T15:55:28Z
dc.date.issued2009-01-28T15:55:28Z
dc.identifier.urihttp://hdl.handle.net/10464/2873
dc.description.abstractSince its discovery nearly a century ago, a-tocopherol (vitamin E) research has been mainly focused on its ability to terminate the cycle of lipid peroxidation in membranes. Nitrobenzoxadiazole fluorescent analogues were made previously to study the intracellular transfer of vitamin E in cells. However, these molecules were reportedly susceptible to photobleaching while under illumination for transfer assays and microscopy. Here is reported the synthesis of a series of fluorescent analogues of vitamin E incorporating the more robust dipyrrometheneboron difluoride fluorophore (BDP-a-Tocs; Aex = 507 nm, Aem = 511 nm). C8-BDP-a-Toc 42c, having an eight-carbon chain between the chromanol and fluorophore, wa<; shown to bind specifically to a-tocopherol transfer protein with a dissociation constant of approximately 100 nM. Another fluorescent analogue of vitamin E with a thienyl derivative of BODIPY that is excited and fluoresces at longer wavelengths (Aex = 561 nm, Aem = 570 nm) is in development.en_US
dc.language.isoengen_US
dc.publisherBrock Universityen_US
dc.subjectVitamin E--Metabolism.en_US
dc.subjectVitamin E--Synthesis.en_US
dc.subjectFluorimetry.en_US
dc.titleSynthesis and characterization of BODIPY-α-tocopherol : a new ligand to explore the intracellular transfer of Vitamin Een_US
dc.typeElectronic Thesis or Dissertationen_US
dc.degree.nameM.Sc. Chemistryen_US
dc.degree.levelMastersen_US
dc.contributor.departmentDepartment of Chemistryen_US
dc.degree.disciplineFaculty of Mathematics and Scienceen_US


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