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dc.contributor.authorZhao, Yuyan.en_US
dc.date.accessioned2010-01-28T15:55:18Z
dc.date.available2010-01-28T15:55:18Z
dc.date.issued2009-01-28T15:55:18Z
dc.identifier.urihttp://hdl.handle.net/10464/2849
dc.description.abstractIn this study, an efficient methodology for the preparation of carbohydrate-RNA conjugates was established, which involved the use of 3,4~diethoxy-3-cyclobutene-l,2- dione (diethyl squarate) as the linking reagent. First, a glycan moiety containing an amino group reacted with diethyl squarate to form an activated glycan, which further reacted with an amino modified oligoribonucleotide to form a glycoconjugate under slightly basic conditions. The effect of glycosylation on the stability of RNA molecules was evaluated on two glycoconjugates, monomannosyl UlO-mer and dimannosyl UlO-mer. In the synthesis of aromatic fluorescent ribosides, perbenzylated ribofuranosyl pyrene and phenanthrene were synthesized from perbenzylated ribolactone. Deprotection of benzyl-protected ribofuranosyl phenanthrene and pyrene by boron tribromide gave ribofuranosyl phenanthrene and ribopyranosyl pyrene, respectively. UV/vis and fluorescent properties of the ribosides were characterized.en_US
dc.language.isoengen_US
dc.publisherBrock Universityen_US
dc.subjectSmall interfering RNA--Synthesis.en_US
dc.titleChemical synthesis of glycosylated oligoribonucleotides for siRNA developmenten_US
dc.typeElectronic Thesis or Dissertationen_US
dc.degree.nameM.Sc. Chemistryen_US
dc.degree.levelMastersen_US
dc.contributor.departmentDepartment of Chemistryen_US
dc.degree.disciplineFaculty of Mathematics and Scienceen_US


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