Show simple item record

dc.contributor.authorSanli, Toran.en_US
dc.date.accessioned2009-11-04T14:55:28Z
dc.date.available2009-11-04T14:55:28Z
dc.date.issued2008-11-04T14:55:28Z
dc.identifier.urihttp://hdl.handle.net/10464/2832
dc.description.abstractExcess plasma free fatty acids (FFA) are correlated with insulin resistance and are a risk factor for the development of type 2 diabetes. In this study we examined the effect of the polyphenol resveratrol on FF A-induced insulin resistance in skeletal muscle cells and the mechanisms involved. Incubation of L6 myotubes with the FF A palmitate significantly decreased the insulin-stimulated glucqse uptake. Importantly, the effect of palmitate was ameliorated by resveratrol. Palmitate significantly increased serine phosphorylation of IRS..; 1 and reduced insulin-stimulated Akt phosphorylation, an effect that was abolished by resveratrol. We then investigated the effect of palmitate and resveratrol on the expression and phosphorylation of JNK, mTOR, p70-S6K, and AMPK kinases. The results demonstrated that our treatments had no effect on the expression of these proteins. However, palmitate increased the phosphorylation of mTOR and p70- S6K, whereas resveratrol abolished this effect and increased the phosphorylation of AMPK. Furthermore, all effects of resveratrol were abolished with sirtuin inhibitors, sirtinol and nicotinamide. These results indicate that resveratrol ameliorated FF A-induced insulin resistance by regulating mTOR and p70-S6K phosphorylation in skeletal muscle cells, through a mechanism involving sirtuins.en_US
dc.language.isoengen_US
dc.publisherBrock Universityen_US
dc.subjectInsulin resistance.en_US
dc.subjectResveratrol--Health aspects.en_US
dc.subjectDiabetes--Treatment.en_US
dc.titleAttenuation of skeletal muscle FFA-induced insulin resistance by the polyphenol resveratrol. Elucidation of the mechanisms involveden_US
dc.typeElectronic Thesis or Dissertationen
dc.degree.nameM.Sc. Applied Health Sciencesen_US
dc.degree.levelMastersen_US
dc.contributor.departmentApplied Health Sciences Programen_US
refterms.dateFOA2021-07-30T01:33:12Z


Files in this item

Thumbnail
Name:
Brock_Sanli_Toran_2008.pdf
Size:
3.636Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record