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dc.contributor.authorLeBlanc, David J.en_US
dc.date.accessioned2009-07-09T18:49:02Z
dc.date.available2009-07-09T18:49:02Z
dc.date.issued1976-07-09T18:49:02Z
dc.identifier.urihttp://hdl.handle.net/10464/2095
dc.description.abstractA study was undertaken' to determine the applicability of gas liquid chromatography to the simultaneous analysis of sugars and sugar phosphates from biological samples. A new method of silylation involving dimethylsulfoxide, hexamethyldisilazane, trimethylchlorosilane and cyclohexane (1:0.2:0.1:1) which rapidly silylated sugars and sugar phosphates was developed. Subsequent chromatography on a 5% SE-52 column gave good resolution of the sugar and sugar phosphate samples. Sugar phosphates decomposed during chromatography and were lost at the 7 x 10-3 ~mole level. Acidic ethanol extraction of yeast samples revealed background contamination from the yeast sample, the culture medium and the silylation reagents which would further limit the level of detection obtainable with the glc for sugars in biological samples to the 3 x 10-4 ~mole level.en_US
dc.language.isoengen_US
dc.publisherBrock Universityen_US
dc.subjectSugars.en_US
dc.subjectSugar phosphates.en_US
dc.subjectGas chromatography--Industrial applications.en_US
dc.subjectSugar--Analysis.en_US
dc.titleThe application of gas liquid chromatography to the simultaneous analysis of sugars and sugar phosphates in biological samples /|nby David J. LeBlanc. -- 260 St. Catharines [Ont. : s. n.],en_US
dc.typeElectronic Thesis or Dissertationen_US
dc.degree.nameM.Sc. Biological Sciencesen_US
dc.degree.levelMastersen_US
dc.contributor.departmentDepartment of Biological Sciencesen_US
dc.degree.disciplineFaculty of Mathematics and Scienceen_US


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