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Immuno-cytochemical localization of glycoproteins involved in recognition and attachment in a mycoparatism

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dc.contributor.author Su, Longcheng. en_US
dc.date.accessioned 2009-07-09T18:38:32Z
dc.date.available 2009-07-09T18:38:32Z
dc.date.issued 1992-07-09T18:38:32Z
dc.identifier.uri http://hdl.handle.net/10464/1907
dc.description.abstract Polyclonal antibodies prepared against the two glycoproteins (Mr 100 and 85 kDa) involved in recognition and attachment of the mycoparasite, Piptocephalis virginiana, to its hosts, Mortierella pusilla and Phascolomyces articulosus, susceptible and resistant, respectively, were employed to localize the antigens at their cell surfaces. Indirect immunocytochemical technique using secondary antibodies labelled with either FITC or gold particles as probes, were used. FITC-Iabelled antibodies revealed a discontinous pattern of fluorescence on the hyphae of MortlerelLa pusilla and no fluorescence on the hyphae of Phascolomyces articulosus. Intensity of fluorescence was high in the germinating spores of both the fungi. Fluoresence could be observed on P. articulosus hyphae pretreated with a commercial proteinase. Fluorescence was not observed on either hyphae or germinating spores of the nonhost M0 r tie re11 a ca ndelabrum and the mycoparasite P. virginiana. Antibodies labelled with gold conjugate showed a different pattern of antigen localization on the hyphal walls of the susceptible and resistant hosts. Patches of gold particles were observed allover the whole cell wall of the susceptible host but only on the inner cell wall layer of the resistant host. Cell wall fragments of the susceptible host but not those of the resistant host, previously incubated with the antibodies inhibited attachment of the mycoparasite. Implications of preferential localization of the antigen in the resistant host and its absence in the nonhost are described. en_US
dc.language.iso eng en_US
dc.publisher Brock University en_US
dc.subject Glycoproteins. en_US
dc.subject Immunocytochemistry. en_US
dc.subject Host-parasite relationships. en_US
dc.subject Parasitism. en_US
dc.title Immuno-cytochemical localization of glycoproteins involved in recognition and attachment in a mycoparatism en_US
dc.type Electronic Thesis or Dissertation en_US
dc.degree.name M.Sc. Biological Sciences en_US
dc.degree.level Masters en_US
dc.contributor.department Department of Biological Sciences en_US
dc.degree.discipline Faculty of Mathematics and Science en_US


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