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dc.contributor.authorFraser, Gordon Andrew Donald.en_US
dc.date.accessioned2009-07-09T17:31:05Z
dc.date.available2009-07-09T17:31:05Z
dc.date.issued1989-07-09T17:31:05Z
dc.identifier.urihttp://hdl.handle.net/10464/1722
dc.description.abstractThis thesis compares the responses of regenerating forelimb tissues of the newt Notophthalmu..f vlridescens to the stresses of hyperthermia and ID.echanical injury of amputation. In particular, both quantitative and qualitative changes in the synthesis of soluble proteins in stump tissues, including those of the heat shock protein family (HSP70-1ike) were examined. Results from SDS-PAGEfluorography indicate that the trauma of amputation mimics the heat shock response both quantitatively and temporally in its transient repression of the synthesis of most normal cellular proteins, and qualitatively. in the locaJized expression of two unique proteins (hsp30 and hsp70). Fluorography of proteins separated by twodimensional gets revealed that thelCl4:alizedt amputation induced 70kDa protein (amp70) was distinct from the more basic newt hsp/hsc70 isoforms. Although limb amputation resulted in an increase in the synthesis of HSP70 mRNA analogous to that induced by heat 3.b.OCKf amp70 did not cross-react with murine monoclonal antibodies directed against both the inducible and cognate HSP70 proteins of the human. Thus, the possible relationship of amp70 to other members of the HSP70-1ike protein family remains unclear. Western analyses indicated that the levels of the constitutive form of HSP70 (hsc70) were found to be regulated in a stage-dependent manner in the distal stump tissues of the regen,erating forelimb of the newt. The highest levels were found in the mid-late bud stage, a period during which rapidly dividing blastema cells begin to redifferentiate in a proximodistal direction. Immediately after amputation) hsc70 synthesis and accumulation was depressed below steady-state levels measured in the unamputated limb~ The results are discussed in light of a possible role for HSPs and amputatio~ induced proteins in the epimorphic regeneration of the amphibian limb.en_US
dc.language.isoengen_US
dc.publisherBrock Universityen_US
dc.subjectNotophthalmus viridescens--Anatomy.en_US
dc.subjectProteins--Synthesis.en_US
dc.subjectArm.en_US
dc.subjectExtremities (Anatomy)--Regeneration.en_US
dc.subjectRegeneration (Biology)en_US
dc.subjectAmputation.en_US
dc.subjectHeat shock proteins.en_US
dc.titleAmputation and heat induced protein synthesis in the regenerating forelimb of Notophthalmus viridescensen_US
dc.typeElectronic Thesis or Dissertationen_US
dc.degree.nameM.Sc. Biological Sciencesen_US
dc.degree.levelMastersen_US
dc.contributor.departmentDepartment of Biological Sciencesen_US
dc.degree.disciplineFaculty of Mathematics and Scienceen_US


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