Digital Repository

A comparative study of in vitro chitin synthase activity in mucoraceous hosts of a mycoparasite

DSpace/Manakin Repository

Show simple item record

dc.contributor.author Begum, Almas. en_US
dc.date.accessioned 2009-07-09T17:30:47Z
dc.date.available 2009-07-09T17:30:47Z
dc.date.issued 1983-07-09T17:30:47Z
dc.identifier.uri http://hdl.handle.net/10464/1698
dc.description.abstract A comparative study of in vitro chitin synthase activity in mucoraceous hosts of a mycoparasite: Chitin synthase, the enzyme responsible for the synthesis of chitin in fungal cell wall was extracted from young hyphae of Choanephora cucurbitarum and Phascolomyces articulosus, susceptible and resistant hosts, respectively, to the mycoparasite, Piptocephalis virginiana. Crude enzyme was identified and characterized by measuring the incorporation of the substrate [14C]-UDP-N-acetylglucosamine, into chitin. Most activity occurred in mixed membrane fraction. Inhibition of activity with Polyoxin D and activation with proteases, N-acetyl-glucosamine and magnesium and other ions was observed. Properties of the crude enzyme preparation such as cofactor requirement, Vmax , apparent Km value for UDP-GlcNAc, inhibition by Polyoxin D, response to pH and to temperature, and stability at 4°C were determined. Enzyme activity from both fungi displayed basically the same features as the corresponding enzymes reported from other mucoraceous fungi. However, the two preparations from P. articulosus and C. cucurbitarum differed from each other in their expressed activity (i.e., the preparations from ~ articulosus exhibited higher latency and higher specific chitin synthase activity than the corresponding preparations from ~ cucurbitarum). Trypsin was effective in activation only over a narrow concentration range. Acid protease was the most effec.tive activator. En.dogenous protease estimation indicated higher protease activity in C. cucurbitarum than in P. articulosus. The suggestion is made that regulation of chitin synthase activities may be related to host resistance in the mycoparasitic system. en_US
dc.language.iso eng en_US
dc.publisher Brock University en_US
dc.subject Host-parasite relationships. en_US
dc.subject Chitin. en_US
dc.subject Fungi. en_US
dc.subject Parasites. en_US
dc.title A comparative study of in vitro chitin synthase activity in mucoraceous hosts of a mycoparasite en_US
dc.type Electronic Thesis or Dissertation en_US
dc.degree.name M.Sc. Biological Sciences en_US
dc.degree.level Masters en_US
dc.contributor.department Department of Biological Sciences en_US
dc.degree.discipline Faculty of Mathematics and Science en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search The Repository


Browse

My Account

Statistics


About the Digital Repository