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The influence of skeletal muscle cell volume on carbohydrate metabolism in contracting skeletal muscle

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dc.contributor.author Cermak, Naomi. en_US
dc.date.accessioned 2009-06-29T14:36:59Z
dc.date.available 2009-06-29T14:36:59Z
dc.date.issued 2006-06-29T14:36:59Z
dc.identifier.uri http://hdl.handle.net/10464/1660
dc.description.abstract This study investigated the regulation of carbohydrate metabolism through changes in skeletal muscle cell volume immediately post contraction and during recovery. Using an established in vitro isolated muscle strip model, soleus (SOL) and extensor digitorum longus (EDL) were dissected from male rats and incubated in an organ bath (perfused with 95% O2; 5% CO2, pH 7.4, temperature 25°C) containing medium- 199 altered to a target osmotic condition (iso-, hypo- or hyper-osmotic; 290, 1 80, 400 mmol/kg). Muscles were stimulated for 10 minutes (40 Hz SOL; 30 Hz EDL) and then either immediately flash frozen or allowed to recover for 20 minutes before subsequent metabolite and enzyme analysis. Results demonstrated a relative water decrease in HYPER vs. HYPOosmotic condition (n=8/group; p<0.05) regardless of muscle type. Specifically, the SOL HYPER condition had elevated metabolite concentrations after 10 minutes of stimulation in comparison to both HYPO and ISO (p<0.05), while EDL muscle did not show any significant difTerences between the HYPER or HYPO conditions. After 20 minutes of recovery, metabolic changes occurred in both SOL and EDL with the SOL HYPER condition showing greater relative changes in metabolite concentrations versus HYPO. The results of the current study have demonstrated that osmotic imbalance induces metabolic change within the skeletal muscle cell and muscle type may influence the mechanisms utilized for cell volume regulation. en_US
dc.language.iso eng en_US
dc.publisher Brock University en_US
dc.subject Carbohydrates en_US
dc.subject Muscle contraction. en_US
dc.title The influence of skeletal muscle cell volume on carbohydrate metabolism in contracting skeletal muscle en_US
dc.type Electronic Thesis or Dissertation en_US
dc.degree.name M.Sc. Applied Health Sciences en_US
dc.degree.level Masters en_US
dc.contributor.department Applied Health Sciences Program en_US


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