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The effect of extracellular osmolality on cell volume and resting skeletal muscle metabolism

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dc.contributor.author Antolic, AnaMaria. en_US
dc.date.accessioned 2009-06-29T14:36:58Z
dc.date.available 2009-06-29T14:36:58Z
dc.date.issued 2006-06-29T14:36:58Z
dc.identifier.uri http://hdl.handle.net/10464/1658
dc.description.abstract The purpose of the current investigation was to establish an in-l'itro skeletal muscle model to study acute alterations in resting skeletal muscle cell volume. Isolated. whole muscle (SOL and EDL) was dissected from Long Evans rats and incubated for 60 min in Sigma Medium-199 (resting tension (lg). bubbled with 95:5% 02:C02, 30 ± 2°C, and pH 7.4). Media osmolality was altered to simulate hypo-osmotic (190 ± 10 Osm) (HYPO) or hyper-osmotic conditions (400 ± 10 Osm) (HYPER) while an iso-osmotic condition (290± 1 0 Osm) (CON) served as a control (n= 17.19.17). Following incubation, relative muscle water content decreased with HYPER and increased with HYPO in both muscle types (p<0.05). The cross-sectional area of HYPO SOL type I and type II fibres increased (p<0.05) while the EDL type 11 fibre area decreased in HYPER and increascd from HYPO exposure. Furthermore, HYPER exposure in both muscles lead to decreased ATP and phosphocreatine (p<0.05) and increased creatine and lactate (p<0.05) compared to CON. This isolated skeletal muscle model proved viable and demonstrated that altering extracellular osmolality could cause acutc alterations in muscle water content and resting muscle metabolism. en_US
dc.language.iso eng en_US
dc.publisher Brock University en_US
dc.subject Osmoregulation. en_US
dc.subject Muscle cells. en_US
dc.subject Muscles en_US
dc.title The effect of extracellular osmolality on cell volume and resting skeletal muscle metabolism en_US
dc.type Electronic Thesis or Dissertation en_US
dc.degree.name M.Sc. Applied Health Sciences en_US
dc.degree.level Masters en_US
dc.contributor.department Applied Health Sciences Program en_US


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