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Effect of citrate on the redox properties of cytochrome C and its kinetic and binding behaviour with cytochrome oxidase /

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dc.contributor.author Brooks, Stephen P. J. en_US
dc.date.accessioned 2009-06-09T18:16:33Z
dc.date.available 2009-06-09T18:16:33Z
dc.date.issued 1981-06-09T18:16:33Z
dc.identifier.uri http://hdl.handle.net/10464/1581
dc.description.abstract Increasing citrate concentration, at constant ionic strength (30 mM) decreases the rate of cytochrome ~ reduction by ascorbate. This effect is also seen at both high (600 mM) and low (19 mM) ionic strengths, and the Kapp for citrate increases with increasing ionic strength. Citrate binds d both ferri -and ferrocytochrome ~, but with a lower affinity for the latter form (Kox . .red d = 2 mM, Kd = 8 mM) as shown by an equilibrium assay with N,N,N',N', Tetramethyl E- phenylenediamine. The reaction of ferricytochrome ~with cyanide is also altered in the presence of citrate: citrate increases the K~PP for cyanide. Column chromatography of cytochrome ~-cytochrome oxidase mixtures shows citrate increases the dissociation constant of the complex. These results are confirmed in kinetic assays for the "loose"site (Km = 20 pM) only. The effect of increasing citrate observable at the "tight" site (Km = 0.25 pM) is on the turnover number and not on the K . These results suggest a mechanism m where anion binding to cytochrome £ at the tight site affects the equilibrium between two forms of cytochrome c bound cytochrome oxidase: an active and an inactive one. en_US
dc.language.iso eng en_US
dc.publisher Brock University en_US
dc.subject Cytochrome c. en_US
dc.subject Oxidation-reduction reaction. en_US
dc.subject Oxidases. en_US
dc.subject Citrates. en_US
dc.title Effect of citrate on the redox properties of cytochrome C and its kinetic and binding behaviour with cytochrome oxidase / en_US
dc.type Electronic Thesis or Dissertation en_US
dc.degree.name M.Sc. Biological Sciences en_US
dc.degree.level Masters en_US
dc.contributor.department Department of Biological Sciences en_US
dc.degree.discipline Faculty of Mathematics and Science en_US


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