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dc.contributor.authorWilkinson, Jennifer
dc.date.accessioned2019-09-11T19:26:39Z
dc.date.available2019-09-11T19:26:39Z
dc.identifier.urihttp://hdl.handle.net/10464/14498
dc.description.abstractSkeletal muscle is a highly active tissue comprising up to 40% of total body weight. This highly dynamic tissue relies on mitochondria for calcium homeostasis, program cell death, and ATP production through mitochondrial respiration. The composition of mitochondrial phospholipids influences the functional efficiency of mitochondrial proteins, and healthy membranes include unsaturated acyl chains to promote respiration. Increasing membrane saturation has been implicated in muscle wasting, a condition caused by apoptosis and identified by the release of specific proteins from the mitochondria. It is unclear how membrane composition promotes the release of these pro-apoptotic proteins, specifically cytochrome c, to induce apoptosis. Thus, in this work synthetic membranes mimicking the composition of mitochondrial membranes from healthy and dystrophic mouse muscle were used. Cytochrome c conformation and function was measured with and without these membranes. There were no differences in protein conformation or function between the healthy and dystrophic membrane mimetics. However, cytochrome c affinity to these membranes was increased with greater unsaturation. This suggests that decreasing membrane saturation, as implicated in muscle wasting diseases, promotes the release of cytochrome c and apoptotic-mediated cell death. As such, interventions to improve or maintain membrane unsaturation may prove to be an alternative therapy for muscle wasting diseases.en_US
dc.language.isoengen_US
dc.publisherBrock Universityen_US
dc.subjectapoptosisen_US
dc.subjectmitochondriaen_US
dc.subjectcardiolipinen_US
dc.subjectbiomembranesen_US
dc.titleThe effect of cardiolipin acyl chain composition on cytochrome c protein conformation and resulting peroxidase activity: Exploring the potential mechanisms that contribute to cellular apoptosis.en_US
dc.typeElectronic Thesis or Dissertationen_US
dc.degree.nameM.Sc. Applied Health Sciencesen_US
dc.degree.levelMastersen_US
dc.contributor.departmentApplied Health Sciences Programen_US
dc.degree.disciplineFaculty of Applied Health Sciencesen_US


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