Bioinformatic and morphological characterization of Catharanthus roseus mutants
dc.contributor.author | Jones, Graham | |
dc.date.accessioned | 2019-06-14T12:39:29Z | |
dc.date.available | 2019-06-14T12:39:29Z | |
dc.identifier.uri | http://hdl.handle.net/10464/14182 | |
dc.description.abstract | Catharanthus roseus, a member of the Apocynaceae family, has been studied extensively for its valuable chemotherapeutic monoterpenoid indole alkaloids (MIAs). Ethyl methanesuphonate (EMS) mutagenesis is a screening tool that has been used to look for altered MIA profiles in hope of discovering mutations of crucial MIA biosynthetic genes. Without a high-throughput mutation detection screen for C. roseus sequencing data, a range of techniques must be used to discover the EMS-induced changes within the plant. Bioinformatic and morphological analysis revealed the likely alterations leading to unique MIA profiles in two C. roseus EMS mutants: the high-ajmalicine accumulating line M2-0754 and the low-MIA accumulating line M2-1582. Expression of geissoschizine synthase (GS) was downregulated almost seven-fold in the leaves of M2-0754, leading to the accumulation of an alternate pathway MIA from the labile intermediate. The low-MIA profile and increased auxin sensitivity of M2-1582 is likely due to the expression of a dysfunctional auxin influx transport protein homologue. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Brock University | en_US |
dc.subject | Catharanthus | en_US |
dc.subject | roseus | en_US |
dc.subject | mutagenesis | en_US |
dc.subject | bioinformatics | en_US |
dc.subject | morphology | en_US |
dc.subject | EMS | en_US |
dc.subject | plant | en_US |
dc.title | Bioinformatic and morphological characterization of Catharanthus roseus mutants | en_US |
dc.type | Electronic Thesis or Dissertation | en |
dc.degree.name | M.Sc. Biotechnology | en_US |
dc.degree.level | Masters | en_US |
dc.contributor.department | Centre for Biotechnology | en_US |
dc.degree.discipline | Faculty of Mathematics and Science | en_US |
refterms.dateFOA | 2021-08-15T01:41:46Z |