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dc.contributor.authorOrmerod, Kiel G.
dc.contributor.authorLePine, Olivia K.
dc.contributor.authorAbbineni, Prabhodh S.
dc.contributor.authorBridgeman, Justin M.
dc.contributor.authorCoorssen, Jens R.
dc.contributor.authorMercier, A. Joffre
dc.contributor.authorTattersall, Glenn J.
dc.date.accessioned2017-05-18T18:01:08Z
dc.date.available2017-05-18T18:01:08Z
dc.date.issued2017-03
dc.identifier.citationOrmerod, K. G., LePine, O. K., Abbineni, P. S., Bridgeman, J. M., Coorssen, J. R., Mercier, A. J., & Tattersall, G. J. (2017). Drosophila development, physiology, behavior, and lifespan are influenced by altered dietary composition. Fly, 1–18. https://doi.org/10.1080/19336934.2017.1304331en_US
dc.identifier.issn1933-6934
dc.identifier.urihttp://hdl.handle.net/10464/12706
dc.description.abstractDiet profoundly influences the behaviour of animals across many phyla. Despite this, most laboratories employing model organisms, such as Drosophila, use multiple, different, commercial or custom-made media for rearing their animals. In addition to measuring growth, fecundity and longevity, we employed several behavioural and physiological assays to determine if and how altering food media influence wild-type (Canton S) Drosophila melanogaster, at larval, pupal, and adult stages. Comparing two commonly used commercial food media we observed several key developmental and morphological differences. Third-instar larvae and pupae developmental timing, body weight and size, and even lifespan significantly differed between the two diets, and some of these differences persisted into adulthood. Diet was also found to produce significantly different thermal preference, locomotory capacity for geotaxis, feeding rates, and lower muscle response to hormonal stimulation. There were no differences, however, in adult thermal preferences, in the number or viability of eggs laid, or in olfactory learning and memory between the diets. We characterized the composition of the two diets and found particularly significant differences in cholesterol and (phospho)lipids between them. Notably, diacylglycerol (DAG) concentrations vary substantially between the two diets, and may contribute to key phenotypic differences, including lifespan. Overall, the data confirm that two different diets can profoundly influence the behaviour, physiology, morphology and development of wild-type Drosophila, with greater behavioural and physiological differences occurring during the larval stages.en_US
dc.language.isoenen_US
dc.publisherTaylor & Francisen_US
dc.subjectdieten_US
dc.subjectD. melanogasteren_US
dc.subjectthermal preferenceen_US
dc.subjectgeotaxisen_US
dc.subjectlearningen_US
dc.subjectmemoryen_US
dc.subjectmorphologyen_US
dc.subjectlocomotionen_US
dc.subjectmuscleen_US
dc.subjectbioactive peptidesen_US
dc.titleDrosophila development, physiology, behaviour, and lifespan are influenced by altered dietary compositionen_US
dc.typeArticleen_US
dc.identifier.doi10.1080/19336934.2017.1304331


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