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Biotransformations of water insoluble substrates in aqueous, two-phase and encapsulated systems /

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dc.contributor.author Iezzi, Diana. en_US
dc.date.accessioned 2009-05-21T12:53:05Z
dc.date.available 2009-05-21T12:53:05Z
dc.date.issued 1999-05-21T12:53:05Z
dc.identifier.uri http://hdl.handle.net/10464/1224
dc.description.abstract The biotransformation of water insoluble substrates by mammalian and bacterial cells has been problematic, since these whole cell reactions are primarily performed in an aqueous environment The implementation of a twophase or encapsulated system has the advantages of providing a low water system along with the physiological environment the cells require to sustain themselves. Encapsulation of mammalian cells by formation of polyamide capsules via interfacial polymerization illustrated that the cells could not survive this type of encapsulation process. Biotransformation of the steroid spironolactone [3] by human kidney carcinoma cells was performed in a substrate-encapsulated system, yielding canrenone [4] in 70% yield. Encapsulation of nitrile-metabolizing Rhodococcus rhodochrous cells using a polyamide membrane yielded leaky capsules, but biotransformation of 2-(4- chlorophenyl)-3-methylbutyronitrile (CPIN) [6] in a free cell system yielded CPIN amide [7] in 40% yield and 94% ee. A two-phase biotransformation of CPIN consisting of a 5:1 ratio of tris buffer, pH 7.2 to octane respectively, gave CPIN acid [8] in 30% yield and 97% ee. It was concluded that Rhodococcus rhodochrous ATCC 17895 contained a nonselective nitrile hydratase and a highly selective amidase enzyme. en_US
dc.language.iso eng en_US
dc.publisher Brock University en_US
dc.subject Biotransformation (Metabolism) en_US
dc.subject Organic compounds en_US
dc.subject Enzymes en_US
dc.subject Microencapsulation. en_US
dc.title Biotransformations of water insoluble substrates in aqueous, two-phase and encapsulated systems / en_US
dc.type Electronic Thesis or Dissertation en_US
dc.degree.name M.Sc. Chemistry en_US
dc.degree.level Masters en_US
dc.contributor.department Department of Chemistry en_US
dc.degree.discipline Faculty of Mathematics and Science en_US


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