Show simple item record

dc.contributor.authorIezzi, Diana.en_US
dc.date.accessioned2009-05-21T12:53:05Z
dc.date.available2009-05-21T12:53:05Z
dc.date.issued1999-05-21T12:53:05Z
dc.identifier.urihttp://hdl.handle.net/10464/1224
dc.description.abstractThe biotransformation of water insoluble substrates by mammalian and bacterial cells has been problematic, since these whole cell reactions are primarily performed in an aqueous environment The implementation of a twophase or encapsulated system has the advantages of providing a low water system along with the physiological environment the cells require to sustain themselves. Encapsulation of mammalian cells by formation of polyamide capsules via interfacial polymerization illustrated that the cells could not survive this type of encapsulation process. Biotransformation of the steroid spironolactone [3] by human kidney carcinoma cells was performed in a substrate-encapsulated system, yielding canrenone [4] in 70% yield. Encapsulation of nitrile-metabolizing Rhodococcus rhodochrous cells using a polyamide membrane yielded leaky capsules, but biotransformation of 2-(4- chlorophenyl)-3-methylbutyronitrile (CPIN) [6] in a free cell system yielded CPIN amide [7] in 40% yield and 94% ee. A two-phase biotransformation of CPIN consisting of a 5:1 ratio of tris buffer, pH 7.2 to octane respectively, gave CPIN acid [8] in 30% yield and 97% ee. It was concluded that Rhodococcus rhodochrous ATCC 17895 contained a nonselective nitrile hydratase and a highly selective amidase enzyme.en_US
dc.language.isoengen_US
dc.publisherBrock Universityen_US
dc.subjectBiotransformation (Metabolism)en_US
dc.subjectOrganic compoundsen_US
dc.subjectEnzymesen_US
dc.subjectMicroencapsulation.en_US
dc.titleBiotransformations of water insoluble substrates in aqueous, two-phase and encapsulated systems /en_US
dc.typeElectronic Thesis or Dissertationen_US
dc.degree.nameM.Sc. Chemistryen_US
dc.degree.levelMastersen_US
dc.contributor.departmentDepartment of Chemistryen_US
dc.degree.disciplineFaculty of Mathematics and Scienceen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record