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The analysis of gene transcripts associated with conidiation in the insect pathogenic fungus, Metarhizium anisopliae /

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dc.contributor.author Small, Cherrie-Lee. en_US
dc.date.accessioned 2009-05-21T12:52:56Z
dc.date.available 2009-05-21T12:52:56Z
dc.date.issued 2004-05-21T12:52:56Z
dc.identifier.uri http://hdl.handle.net/10464/1202
dc.description.abstract Conidia of the insect pathogenic fungus, Metarhizium anisopliae play an important role in pathogenicity because they are the infective propagules that adhere to the surface of the insect, then germinate and give rise to hyphal penetration of the insect cuticle. Conidia are produced in the final stages of insect infection as the mycelia emerge from the insect cadaver. The genes associated with conidiation have not yet been studied in this fiingus. hi this study we used the PCR-based technique, suppression subtractive hybridization (SSH) to selectively amplify conidial-associated genes in M. anisopliae. We then identified the presence of these differentially expressed genes using the National Center for Biotechnology Information database. One of the transcripts encoded an extracellular subtilisin-like protease, Prl, which plays a fundamental role in cuticular protein degradation. Analysis of the patterns of gene expression of the transcripts using RT-PCR indicated that conidial-associated cDNAs are expressed during the development of the mature conidium. RT-PCR analysis was also performed to examine in vivo expression of Prl during infection of waxworm larvae {Galleria mellonelld). Results showed expression of Prl as mycelia emerge and produce conidia on the surface of the cadaver. It is well documented that Prl is produced during the initial stages of transcuticular penetration by M. anisopliae. We suggest that upregulation of Prl is part of the mechanism by which reverse (from inside to the outside of the host) transcuticular penetration of the insect cuticle allows subsequent conidiation on the cadaver. en_US
dc.language.iso eng en_US
dc.publisher Brock University en_US
dc.subject Molecular genetics. en_US
dc.subject Conidia. en_US
dc.subject Metarhizium anisopliae. en_US
dc.subject Entomopathogenic fungi. en_US
dc.title The analysis of gene transcripts associated with conidiation in the insect pathogenic fungus, Metarhizium anisopliae / en_US
dc.type Electronic Thesis or Dissertation en_US
dc.degree.name M.Sc. Biological Sciences en_US
dc.degree.level Masters en_US
dc.contributor.department Department of Biological Sciences en_US
dc.degree.discipline Faculty of Mathematics and Science en_US


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